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Determination of 53 prohibited veterinary drug residues in goat horn by salting-out assisted liquid-liquid extraction combined with UHPLC-MS/MS
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Bai-hao LAO1, 2, Jian SUN2, *, Jing-xian ZHANG2, Hong YU2, Ying-ying RAN2, Fan HUANG2, Shen JI1, 2, Qing HU1, 2, *
Acta Pharmaceutica Sinica | 2025, 60(3) : 791 - 800
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Acta Pharmaceutica Sinica | 2025, 60(3): 791-800
• Original Articles •
Determination of 53 prohibited veterinary drug residues in goat horn by salting-out assisted liquid-liquid extraction combined with UHPLC-MS/MS
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Bai-hao LAO1, 2, Jian SUN2, *, Jing-xian ZHANG2, Hong YU2, Ying-ying RAN2, Fan HUANG2, Shen JI1, 2, Qing HU1, 2, *
Affiliations
  • 1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. National Medical Products Administration Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, Shanghai 201203, China
Published: 2025-03-12 doi: 10.16438/j.0513-4870.2024-0995
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A detection method using UHPLC-MS/MS was established for the determination of 53 prohibited veterinary drug residues in goat horns, including 16 tranquilizers, 14 β-agonists, chloramphenicol, 4 fluoroquinolones, 7 nitroimidazoles, 3 quinoxalines, and 8 other compounds. Samples were extracted using salting-out assisted liquid-liquid extraction (SALLE). A Zorbax Eclipse Plus C18 column (1.8 μm, 3.0 mm × 150 mm, Agilent) was used with 0.1% formic acid solution-acetonitrile as the mobile phase for gradient elution. Detection was performed in positive and negative electrospray ionization modes using multiple reaction monitoring (MRM). All 53 analytes showed good linearity within their respective concentration ranges, with correlation coefficients above 0.99. The average recoveries at three spiked levels ranged from 70.4% to 118.7%, with relative standard deviations (RSDs) ranging from 0.69% to 12.07%. The limits of detection (LODs) ranged from 0.1 to 50 μg·kg-1, and the limits of quantitation (LOQs) ranged from 0.2 to 100 μg·kg-1. This method has been applied to the determination of real samples.

goat horn  /  prohibited veterinary drug residue  /  salting-out assisted liquid-liquid extraction  /  UHPLC-MS/MS
Bai-hao LAO, Jian SUN, Jing-xian ZHANG, Hong YU, Ying-ying RAN, Fan HUANG, Shen JI, Qing HU. Determination of 53 prohibited veterinary drug residues in goat horn by salting-out assisted liquid-liquid extraction combined with UHPLC-MS/MS[J]. Acta Pharmaceutica Sinica, 2025 , 60 (3) : 791 -800 . DOI: 10.16438/j.0513-4870.2024-0995
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Year 2025 volume 60 Issue 3
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Article Info
doi: 10.16438/j.0513-4870.2024-0995
  • Receive Date：2024-10-15
  • Online Date：2025-11-06
  • Published：2025-03-12
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History
  • Received：2024-10-15
  • Revised：2024-12-15
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Affiliations
    1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. National Medical Products Administration Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, Shanghai 201203, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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