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Preliminary study on the mechanism of halofuginone inhibiting the activity of HepG2 cells
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Meng-yang CHEN1, 2, Rui-ping HUAI1, 2, Dan-ni YANG1, 2, Li-jie LEI1, 2, Qiu-lin PU1, 2, Li-li XIONG2, *
Acta Pharmaceutica Sinica | 2024, 59(2) : 368 - 373
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Acta Pharmaceutica Sinica | 2024, 59(2): 368-373
• Original Articles •
Preliminary study on the mechanism of halofuginone inhibiting the activity of HepG2 cells
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Meng-yang CHEN1, 2, Rui-ping HUAI1, 2, Dan-ni YANG1, 2, Li-jie LEI1, 2, Qiu-lin PU1, 2, Li-li XIONG2, *
Affiliations
  • 1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610000, China
  • 2. School of Chemistry, Southwest Jiaotong University, Chengdu 610000, China
Published: 2024-02-12 doi: 10.16438/j.0513-4870.2023-0216
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This study aimed to investigate halofuginone's inhibitory effect and mechanism on the activity of hepatocellular carcinoma cells. HepG2 cells were used to detect the effects of halofuginone. After treatment, cell activity, cell migration, cell cycle, and cell apoptosis were detected by CCK-8, transwell, and flow cytometry, respectively. The expression levels of growth and metabolism-related factors such as citrate synthase (CS), ketoglutarate dehydrogenase (OGDH), and isocitrate deoxygenase (IDH) were detected by real-time quantitative PCR and Western blot. Compared with the control group, the activity of HepG2 cells was significantly inhibited by halofuginone (P < 0.01), the migration rate of HepG2 cells was decreased (P < 0.01), the apoptosis of HepG2 cells was induced (P < 0.01), and the cell cycle was arrested in S phase (P < 0.01). The expression levels of tricarboxylic acid key enzymes CS, IDH3, and OGDH were up-regulated, the expression level of isocitrate dehydrogenase isoenzymes IDH1 and IDH2 were down-regulation. In conclusion, halofuginone can inhibit the proliferation and migration of HepG2 cells and promote apoptosis in a dose-dependent manner, which may be due to the promotion of the aerobic metabolism of cells.

halofuginone  /  tricarboxylic acid  /  HepG2 cell  /  isocitrate dehydrogenase  /  aerobic metabolism
Meng-yang CHEN, Rui-ping HUAI, Dan-ni YANG, Li-jie LEI, Qiu-lin PU, Li-li XIONG. Preliminary study on the mechanism of halofuginone inhibiting the activity of HepG2 cells[J]. Acta Pharmaceutica Sinica, 2024 , 59 (2) : 368 -373 . DOI: 10.16438/j.0513-4870.2023-0216
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Year 2024 volume 59 Issue 2
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Article Info
doi: 10.16438/j.0513-4870.2023-0216
  • Receive Date：2023-02-22
  • Online Date：2025-11-28
  • Published：2024-02-12
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History
  • Received：2023-02-22
  • Revised：2023-06-13
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    1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610000, China
    2. School of Chemistry, Southwest Jiaotong University, Chengdu 610000, China
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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