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Preparation and preliminary evaluation of SARS-CoV-2 DNA vaccine based on PLGA nanoparticles
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Ya-qi WU1, 2, Meng LI2, Hao-nan XING2, Da-quan CHEN1, *, Ai-ping ZHENG2, *
Acta Pharmaceutica Sinica | 2023, 58(6) : 1677 - 1684
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Acta Pharmaceutica Sinica | 2023, 58(6): 1677-1684
• Original Articles •
Preparation and preliminary evaluation of SARS-CoV-2 DNA vaccine based on PLGA nanoparticles
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Ya-qi WU1, 2, Meng LI2, Hao-nan XING2, Da-quan CHEN1, *, Ai-ping ZHENG2, *
Affiliations
  • 1. Pharmacy School, Yantai University, Yantai 264005, China
  • 2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
Published: 2023-06-12 doi: 10.16438/j.0513-4870.2022-1347
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We constructed and optimized the plasmid DNA (pDNA) Opt-S encoding the gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein, using poly (lactic-co-glycolic acid) copolymer (PLGA) as a delivery carrier for pDNA. PLGA-pDNA NPs were loaded by nanoprecipitation and its properties in vitro were preliminary evaluated. The results showed that the prepared PLGA-pDNA NPs were regular morphology, clear edges, with an average particle size of (184.2 ± 2.4) nm, polydisperse index (PDI) of 0.093 ± 0.013, zeta potential of (-68.10 ± 0.36) mV, and encapsulation rate of (98.92 ± 0.22)%. The PLGA-pDNA NPs were stable at -20 ℃ for 7 months and could protect pDNA against nuclease degradation. And they also exhibited sustained release of pDNA in vitro. The PLGA-pDNA NPs have low cytotoxicity and high safety. In addition, in vitro transfection experiments showed that the SARS-CoV-2 S gene could enter cells and be expressed. These results indicate that PLGA-pDNA NPs non-viral gene vector have simple preparation process and good performance, which are expected to provide a new idea for the research and development of SARS-CoV-2 vaccine.

corona virus disease 2019  /  SARS-CoV-2 DNA vaccine  /  poly (lactic-co-glycolic acid) copolymer  /  nanoprecipitation method  /  non-viral gene carrier
Ya-qi WU, Meng LI, Hao-nan XING, Da-quan CHEN, Ai-ping ZHENG. Preparation and preliminary evaluation of SARS-CoV-2 DNA vaccine based on PLGA nanoparticles[J]. Acta Pharmaceutica Sinica, 2023 , 58 (6) : 1677 -1684 . DOI: 10.16438/j.0513-4870.2022-1347
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doi: 10.16438/j.0513-4870.2022-1347
  • Receive Date：2022-12-07
  • Online Date：2025-11-21
  • Published：2023-06-12
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  • Received：2022-12-07
  • Revised：2023-03-08
Affiliations
    1. Pharmacy School, Yantai University, Yantai 264005, China
    2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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