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Recent progress on detection techniques for human hormones
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Peng-yue SONG1, 2, Run-ran MA1, Jia-bo WANG1, *, Wei-jun KONG1, *
Acta Pharmaceutica Sinica | 2023, 58(5) : 1232 - 1244
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Acta Pharmaceutica Sinica | 2023, 58(5): 1232-1244
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Recent progress on detection techniques for human hormones
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Peng-yue SONG1, 2, Run-ran MA1, Jia-bo WANG1, *, Wei-jun KONG1, *
Affiliations
  • 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Published: 2023-05-12 doi: 10.16438/j.0513-4870.2022-1332
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Human hormones at trace levels play a vital role in the regulation of a variety of functions and systems in the body, and an imbalance in hormone levels can lead to the emergence and development of diverse diseases. Therefore, the development of reliable sample pretreatment methods and sensitive and accurate analytical techniques for human hormone detection could contribute to the prevention, diagnosis and treatment of diseases, providing significant improvement for human health. Human samples which are usually used to detecting hormones, such as blood, saliva, urine and other matrix are more complex, so sample pretreatment is an important step to ensure the accuracy and reliability in the detection of hormones. In this review three common sample pretreatment methods including solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PP) methods are discussed. Then, recent research progress in conventional techniques like liquid/gas chromatography and liquid/gas chromatography-mass spectrometry (LC/GC-MS/MS), as well as some novel strategies, such as immunoassay including chemiluminescence immunoassay (CLIA), lateral-flow immunoassay (LFIA) and time-resolved fluoroimmunoassay (TRFIA), and sensor technology including electrochemical (EC), fluorescent (FL) and surface-enhanced Raman scattering (SERS) sensors, and microfluidic chip analysis are discussed for human hormone detection. Finally, the future perspective on the use of these methods for hormone detection is considered. It is hoped to provide powerful insights to researchers for the relevant researches.

human hormone  /  sample pretreatment  /  immunoassay  /  sensor  /  microfluidic chip
Peng-yue SONG, Run-ran MA, Jia-bo WANG, Wei-jun KONG. Recent progress on detection techniques for human hormones[J]. Acta Pharmaceutica Sinica, 2023 , 58 (5) : 1232 -1244 . DOI: 10.16438/j.0513-4870.2022-1332
Year 2023 volume 58 Issue 5
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Article Info
doi: 10.16438/j.0513-4870.2022-1332
  • Receive Date:2022-12-05
  • Online Date:2025-11-21
  • Published:2023-05-12
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  • Received:2022-12-05
  • Revised:2023-02-10
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    1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
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Genus
种数
Number of
species
占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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