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A novel O-methyltransferase with substrate promiscuity from Ardisia japonica
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Qi LIU1, Rui-qi YAN1, Bing-han XIE1, Song-yang SUI2, Ke-bo XIE2, Jun-gui DAI2, *, Lin YANG1, *
Acta Pharmaceutica Sinica | 2023, 58(5) : 1372 - 1382
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Acta Pharmaceutica Sinica | 2023, 58(5): 1372-1382
• Original Articles •
A novel O-methyltransferase with substrate promiscuity from Ardisia japonica
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Qi LIU1, Rui-qi YAN1, Bing-han XIE1, Song-yang SUI2, Ke-bo XIE2, Jun-gui DAI2, *, Lin YANG1, *
Affiliations
  • 1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
  • 2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, CAMS Key Laboratory of Enzymes and Natural Drug Biocatalysis, NHC Key Laboratory of Natural Drug Biosynthesis, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China
Published: 2023-05-12 doi: 10.16438/j.0513-4870.2022-1102
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O-methyltransferases (OMTs) are one of the key tailoring enzymes in the biosynthesis of many natural products, O-methylation can not only reduce the reactivity of natural products, but also alter their solubility, stability and biological activities. Based on the transcriptome data of Ardisia japonica, a full-length cDNA sequence of candidate OMT (termed as AjOMT1) was cloned by reverse transcription-polymerase chain reaction (RT-PCR) and expressed in Escherichia coli (E. coli) for the first time. In vitro enzyme catalytic activity assay showed that the recombinant AjOMT1 could effectively catalyze quercetin to form O-methylated products. Most importantly, AjOMT1 showed unprecedented substrate promiscuity towards structurally various compounds including flavonoids, stilbenes, coumarins, alkaloids and phenylpropanoids, especially preferring to the compounds with adjacent phenolic hydroxyl groups, and showed regio-selectivity. These results suggested that AjOMT1 could be used as the tool enzyme to conduct O-methylation of different types of compounds to produce diverse active methylated products, and provide a new method for drug discovery, even universal part for synthetic biology of natural products.

Ardisia japonica  /  O-methyltransferase  /  O-methylation  /  substrate promiscuity  /  regio-selectivity
Qi LIU, Rui-qi YAN, Bing-han XIE, Song-yang SUI, Ke-bo XIE, Jun-gui DAI, Lin YANG. A novel O-methyltransferase with substrate promiscuity from Ardisia japonica[J]. Acta Pharmaceutica Sinica, 2023 , 58 (5) : 1372 -1382 . DOI: 10.16438/j.0513-4870.2022-1102
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doi: 10.16438/j.0513-4870.2022-1102
  • Receive Date：2022-10-10
  • Online Date：2025-11-21
  • Published：2023-05-12
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  • Received：2022-10-10
  • Revised：2022-11-10
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Affiliations
    1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
    2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, CAMS Key Laboratory of Enzymes and Natural Drug Biocatalysis, NHC Key Laboratory of Natural Drug Biosynthesis, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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