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In vitro inhibition of carnosic acid against influenza A virus infections
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Hai-ying PENG, Ze-xin LIU, Xia YANG, Dian QIU, Wei-xin JIA, Wen-bao QI, Jian-xin CHEN*, Li WU*
Acta Pharmaceutica Sinica | 2023, 58(2) : 360 - 370
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Acta Pharmaceutica Sinica | 2023, 58(2): 360-370
• Original Articles •
In vitro inhibition of carnosic acid against influenza A virus infections
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Hai-ying PENG, Ze-xin LIU, Xia YANG, Dian QIU, Wei-xin JIA, Wen-bao QI, Jian-xin CHEN*, Li WU*
Affiliations
  • College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Published: 2023-02-12 doi: 10.16438/j.0513-4870.2022-0705
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Carnosic acid (CA) is the main phenolic diterpenoid active ingredient in plants such as rosemary and sage, and has antiviral, antioxidant, anti-inflammatory effects and so on, however, its antiviral activity against influenza virus infections was not reported. In this study, antiviral activities against influenza A virus infections of three main bioactive ingredients from rosemary, including rosmarinic acid, CA and ursolic acid, were evaluated using virus titer titration assay, and CA showed remarkable inhibition on influenza H5N1 replication in A549 cells. The antiviral activity of CA was further confirmed and its mechanism of action was investigated using the indirect immunofluorescence assay (IFA), Western blot and real-time fluorescence quantification polymerase chain reaction (qRT-PCR). The results showed that the 50% effective concentration (EC50) of CA against influenza H5N1 in A549 cells and MDCK cells were 4.30 and 3.64 μmol·L-1, respectively. Meanwhile, CA also showed inhibition on influenza virus 2009panH1N1 (EC50: 10.1 μmol·L-1) and H3N2 (EC50: 12.8 μmol·L-1) replications in A549 cells. Mechanistic studies showed that antiviral activity of CA is related to its induction of heme oxygenase-1 (HO-1) in A549 cells and suppression on production of reactive oxygen in H5N1-infected cells.

carnosic acid  /  influenza virus  /  antiviral activity  /  heme oxygenase-1 signaling pathway  /  reactive oxygen species
Hai-ying PENG, Ze-xin LIU, Xia YANG, Dian QIU, Wei-xin JIA, Wen-bao QI, Jian-xin CHEN, Li WU. In vitro inhibition of carnosic acid against influenza A virus infections[J]. Acta Pharmaceutica Sinica, 2023 , 58 (2) : 360 -370 . DOI: 10.16438/j.0513-4870.2022-0705
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Year 2023 volume 58 Issue 2
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doi: 10.16438/j.0513-4870.2022-0705
  • Receive Date：2022-06-08
  • Online Date：2025-11-21
  • Published：2023-02-12
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  • Received：2022-06-08
  • Revised：2022-08-20
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    College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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