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Cloning and analysis of STR gene and its promoter from Uncaria
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Hao ZHOU, Xing-xing LU, Wen-wen AO, Hai-min LIAO, Ming-sheng ZHANG, Wei QIANG*
Acta Pharmaceutica Sinica | 2022, 57(5) : 1526 - 1536
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Acta Pharmaceutica Sinica | 2022, 57(5): 1526-1536
• Original Articles •
Cloning and analysis of STR gene and its promoter from Uncaria
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Hao ZHOU, Xing-xing LU, Wen-wen AO, Hai-min LIAO, Ming-sheng ZHANG, Wei QIANG*
Affiliations
  • Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
Published: 2022-05-12 doi: 10.16438/j.0513-4870.2021-1700
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On the basis of the Uncaria transcriptome, specific primers were designed for UrSTR. The full-length cDNA of UrSTR (GeneBank: OL310251) was 1 541 bp, encoding 345 amino acid residues, and the promoter region sequence of UrSTR (GeneBank: OL310252) was 1 179 bp. Phylogenetic tree is revealed that UrSTR had a closest relationship with STR from Ophiorrhiza pumila and Ophiorrhiza japonica. Localization of UrSTR protein is revealed located in the vacuole membrane. Plant-care analysis indicated that the promoter region sequence of UrSTR, covering multiple light, stress and hormone-response cis-regulatory elements, and verified transcriptional activity. The results of SDS-PAGE show that pET-28a-UrSTR recombinant protein was successfully expressed, and the size was anticipated. The UrSTR prokaryotic expression system needs to be optimized in the later stage. The research lays the foundation for further purification to study its structure and functional characterization of the UrSTR protein.

Uncaria rhynchophylla  /  strictosidine synthase  /  promoter  /  subcellular localization  /  prokaryotic expression
Hao ZHOU, Xing-xing LU, Wen-wen AO, Hai-min LIAO, Ming-sheng ZHANG, Wei QIANG. Cloning and analysis of STR gene and its promoter from Uncaria[J]. Acta Pharmaceutica Sinica, 2022 , 57 (5) : 1526 -1536 . DOI: 10.16438/j.0513-4870.2021-1700
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doi: 10.16438/j.0513-4870.2021-1700
  • Receive Date：2021-11-01
  • Online Date：2025-12-23
  • Published：2022-05-12
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  • Received：2021-11-01
  • Revised：2021-12-28
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Affiliations
    Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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