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Identification and functional characterization of 2, 3-oxidosqualene cyclase genes family in Tripterygium wilfordii
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Yuan LIU1, Li-chan TU2, Yun LU1, Meng XIA1, Wei GAO1, 3, *
Acta Pharmaceutica Sinica | 2021, 56(12) : 3370 - 3376
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Acta Pharmaceutica Sinica | 2021, 56(12): 3370-3376
• Special Reports Ⅱ: Biosynthesis of Plant Natural Products and Synthetic Biology for Their Production •
Identification and functional characterization of 2, 3-oxidosqualene cyclase genes family in Tripterygium wilfordii
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Yuan LIU1, Li-chan TU2, Yun LU1, Meng XIA1, Wei GAO1, 3, *
Affiliations
  • 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • 2. School of Medicine, Zhejiang University City College, Hangzhou 310015, China
  • 3. Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
Published: 2021-12-12 doi: 10.16438/j.0513-4870.2021-1628
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Tripterygium wilfordii Hook. f. is a valuable medicinal plant, with anti-tumor, anti-inflammatory, immunosuppressive and other pharmacological activities. Triterpenoids are one of the main active components that exert pharmacological effects. However, the content of triterpenoids dominated by triptolide is very low in Tripterygium wilfordii, and the analysis of the biosynthetic pathway of triterpenoids in Tripterygium wilfordii provides an effective new idea for obtaining these compounds. 2, 3-Oxidosqualene cyclases (OSCs) are the key enzyme that catalyzes the formation of triterpene skeleton diversity. Based on the genome and transcriptome data of Tripterygium wilfordii, 16 OSC genes were identified and analyzed. Phylogenetic analysis showed that 16 TwOSC proteins could be mainly classified as four groups. They are β-amyrin synthase group, friedelin synthase group, multifunctional amyrin synthase and cycloartenol synthase group. TwOSC6 was successfully cloned. Functional characterization analysis revealed that TwOSC6 can catalyze the formation of α-amyrin and β-amyrin. This indicates that TwOSC6 is a multifunctional amyrin synthase. This provides new gene resources for the diversity of Tripterygium wilfordii triterpenoids, as well as new gene elements for biosynthesis triterpenoids.

Tripterygium wilfordii  /  2, 3-oxidosqualene cyclase  /  triterpenoid  /  α-amyrin  /  function characterization
Yuan LIU, Li-chan TU, Yun LU, Meng XIA, Wei GAO. Identification and functional characterization of 2, 3-oxidosqualene cyclase genes family in Tripterygium wilfordii[J]. Acta Pharmaceutica Sinica, 2021 , 56 (12) : 3370 -3376 . DOI: 10.16438/j.0513-4870.2021-1628
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Year 2021 volume 56 Issue 12
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doi: 10.16438/j.0513-4870.2021-1628
  • Receive Date：2021-11-15
  • Online Date：2025-12-18
  • Published：2021-12-12
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  • Received：2021-11-15
  • Revised：2021-12-06
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Affiliations
    1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    2. School of Medicine, Zhejiang University City College, Hangzhou 310015, China
    3. Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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