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Baicalein inhibits neuroinflammation via NOX2/STAT1/NF-κB pathway in LPS-induced BV-2 cells based on bioinformatics methods
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Wu-yan YANG1, 2, 3, Jiao-jiao YAN1, 2, 3, Li GAO1, 2, 3, *, Xue-mei QIN1, 2, 3, *
Acta Pharmaceutica Sinica | 2022, 57(3) : 716 - 723
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Acta Pharmaceutica Sinica | 2022, 57(3): 716-723
• Original Articles •
Baicalein inhibits neuroinflammation via NOX2/STAT1/NF-κB pathway in LPS-induced BV-2 cells based on bioinformatics methods
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Wu-yan YANG1, 2, 3, Jiao-jiao YAN1, 2, 3, Li GAO1, 2, 3, *, Xue-mei QIN1, 2, 3, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
  • 3. The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
Published: 2022-03-12 doi: 10.16438/j.0513-4870.2021-1339
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This study identified the exact molecular mechanisms of baicalein on neuroinflammation in lipopolysaccharide (LPS)-induced BV-2 cells. Bioinformatics methods and molecular docking were integrated for predicting the potential targets and mechanisms of baicalein. Immunofluorescence staining and Western blot were used to analyze the predicted key targets [inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)], the expression level of protein related to signal transducer and activator of transcription 1/nuclear factor kappa-B (STAT1/NF- κB) signaling pathway and its upstream regulator NADPH oxidase-2 (NOX2), and then the mechanism of baicalein in alleviating neuroinflammation was explored. The results showed that iNOS and COX-2 were predicted as the key targets and NF-κB signaling pathway was one of the important pathways by bioinformatics methods and molecular docking. Experimental verification showed that baicalein could significantly reduce the expression of iNOS and COX-2, inhibit the phosphorylation of NF-κB and STAT1 and the production of NOX2 in LPS-induced BV-2 cells. To sum up, baicalein could effectively inhibit the inflammatory reaction in LPS-induced BV-2 cells through regulating NOX2 (gp91phox/p47phox)/STAT1/NF-κB pathway.

baicalein  /  neuroinflammation  /  network pharmacology  /  molecular docking  /  mechanism
Wu-yan YANG, Jiao-jiao YAN, Li GAO, Xue-mei QIN. Baicalein inhibits neuroinflammation via NOX2/STAT1/NF-κB pathway in LPS-induced BV-2 cells based on bioinformatics methods[J]. Acta Pharmaceutica Sinica, 2022 , 57 (3) : 716 -723 . DOI: 10.16438/j.0513-4870.2021-1339
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Article Info
doi: 10.16438/j.0513-4870.2021-1339
  • Receive Date：2021-09-13
  • Online Date：2025-12-22
  • Published：2022-03-12
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History
  • Received：2021-09-13
  • Revised：2021-10-15
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Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
    3. The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
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