Home Journals Articles Updates About
CN
image
收藏切换
Controlled release and cytobiology of recombinant human parathyroid hormone 1-34 mediated by hyaluronic acid thermo-sensitive hydrogels
收藏切换
PDF
Shao-yan XUAN1, Yue ZHU2, 3, Lin ZHANG1, Zhi-hua TANG1, Feng XU2, Teng CHEN2, Zu-hua WANG2, 3, *
Acta Pharmaceutica Sinica | 2022, 57(3) : 809 - 817
Less
收藏切换
Acta Pharmaceutica Sinica | 2022, 57(3): 809-817
• Original Articles •
Controlled release and cytobiology of recombinant human parathyroid hormone 1-34 mediated by hyaluronic acid thermo-sensitive hydrogels
Full
Shao-yan XUAN1, Yue ZHU2, 3, Lin ZHANG1, Zhi-hua TANG1, Feng XU2, Teng CHEN2, Zu-hua WANG2, 3, *
Affiliations
  • 1. Shaoxing People's Hospital, Shaoxing 312000, China
  • 2. Nano-drug Technology Research Center, College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
  • 3. Guizhou Engineering Technology Research Center for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China
Published: 2022-03-12 doi: 10.16438/j.0513-4870.2021-0999
Outline
收藏切换

The bone formation promoter recombinant human parathyroid hormone 1-34 [PTH (1-34)] has a short half-life and low bioavailability. In this study, we prepared a biodegradable and temperature-sensitive hyaluronic acid-poly-N-isopropyl acrylamide (AHA-g-PNIPAAm), and further investigated its effects of PTH (1-34) release and cell behavior as drug carrier. The structure of AHA-g-PNIPAAM was confirmed by hydrogen nuclear magnetic resonance spectroscopy and infrared spectroscopy. Next, PTH (1-34) loaded thermo-sensitive hydrogels were prepared by physical swelling method and their stability was investigated. The morphology of hydrogel was observed by scanning electron microscope. The minimum critical transition temperature and drug release behavior of hydrogels were investigated by ultraviolet spectrophotometry. The tetrazolium-based colorimetric assay (MTT assay) was used to investigate the toxicity and proliferation effects of PTH (1-34)-loaded thermo-sensitive hydrogel on mouse mononuclear macrophage RAW264.7 and mouse precranial osteoblasts MC3T3-E1. The effect of PTH (1-34)-loaded thermo-sensitive hydrogel on the differentiation of RAW264.7 was investigated by the tartrate-resistant acid phosphatase assay. The results showed that the PTH (1-34)-loaded thermo-sensitive hydrogel prepared in this study displayed regular three-dimensional honeycomb structure, and had good stability, thermo-sensitivity and sustained and controlled release properties, which could promote the proliferation of MC3T3-E1 cells more effectively and inhibit the differentiation of RAW264.7 into osteoclasts.

hyaluronic acid  /  thermo-sensitive hydrogel  /  drug sustained and controlled release  /  osteoclast  /  recombinant human parathyroid hormone (1-34)
Shao-yan XUAN, Yue ZHU, Lin ZHANG, Zhi-hua TANG, Feng XU, Teng CHEN, Zu-hua WANG. Controlled release and cytobiology of recombinant human parathyroid hormone 1-34 mediated by hyaluronic acid thermo-sensitive hydrogels[J]. Acta Pharmaceutica Sinica, 2022 , 57 (3) : 809 -817 . DOI: 10.16438/j.0513-4870.2021-0999
Appendix
Less
Year 2022 volume 57 Issue 3
PDF
119
50
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-0999
  • Receive Date：2021-07-06
  • Online Date：2025-12-22
  • Published：2022-03-12
Article Data
Affiliations
History
  • Received：2021-07-06
  • Revised：2021-08-05
Funding
Affiliations
    1. Shaoxing People's Hospital, Shaoxing 312000, China
    2. Nano-drug Technology Research Center, College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
    3. Guizhou Engineering Technology Research Center for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine, Guiyang 550025, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-0999
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House