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Effect of DC vaccine sensitized with CpG ODN combined with tumor antigen on murine melanoma
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Jia-xin ZENG1, 2, Wen-yuan ZHANG2, Er-gang LIU3, *, Ling-yun LIU4, Yong-zhuo HUANG2, 3, Hui-yuan WANG2, *
Acta Pharmaceutica Sinica | 2022, 57(2) : 385 - 391
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Acta Pharmaceutica Sinica | 2022, 57(2): 385-391
• Original Articles •
Effect of DC vaccine sensitized with CpG ODN combined with tumor antigen on murine melanoma
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Jia-xin ZENG1, 2, Wen-yuan ZHANG2, Er-gang LIU3, *, Ling-yun LIU4, Yong-zhuo HUANG2, 3, Hui-yuan WANG2, *
Affiliations
  • 1. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
  • 2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • 3. Zhongshan Branch, the Institute of Drug Research and Development, Chinese Academy of Sciences, Zhongshan 528437, China
  • 4. The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Published: 2022-02-12 doi: 10.16438/j.0513-4870.2021-0760
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The potential application of dendritic cells (DC) sensitized with cytosine-phosphoric acid-guanine (CpG) oligodeoxynucleotide (ODN) and tumor antigen as a vaccine against murine melanoma was investigated with freshly isolated mouse bone marrow-derived dendritic cells. For the DC vaccine preparation, DC were sensitized with the B16 tumor antigen and CpG ODN was used to promote further maturation of the DC. The immunogenic activity of the vaccine was evaluated in vitro by determining the proliferation of T lymphocytes and the killing effect of cytotoxic T lymphocytes (CTL) on B16 tumor cells. The DC vaccine was injected intraperitoneally and tumor inhibition in mice bearing B16 xenografts was examined. All mice were cared for under an approved SIMM Institutional Animal Care and Use Committee (IACUC) protocol. In vitro, this DC vaccine promoted the proliferation of T lymphocytes and showed a potent killing effect on the target B16 cells. In vivo experiments showed that after treatment or pre-immunization both the tumor volume and weight were significantly decreased. The DC vaccine with CpG ODN and tumor antigen exhibited an inhibitory effect against melanoma, providing a potential method for melanoma cancer treatment.

dendritic cell  /  tumor vaccine  /  CpG oligodeoxynucleotide  /  melanoma  /  immunotherapy
Jia-xin ZENG, Wen-yuan ZHANG, Er-gang LIU, Ling-yun LIU, Yong-zhuo HUANG, Hui-yuan WANG. Effect of DC vaccine sensitized with CpG ODN combined with tumor antigen on murine melanoma[J]. Acta Pharmaceutica Sinica, 2022 , 57 (2) : 385 -391 . DOI: 10.16438/j.0513-4870.2021-0760
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Article Info
doi: 10.16438/j.0513-4870.2021-0760
  • Receive Date：2021-05-18
  • Online Date：2025-12-22
  • Published：2022-02-12
Article Data
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History
  • Received：2021-05-18
  • Revised：2021-07-02
Funding
Affiliations
    1. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
    3. Zhongshan Branch, the Institute of Drug Research and Development, Chinese Academy of Sciences, Zhongshan 528437, China
    4. The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
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表12种不同金属材料的力学参数
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Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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