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Molecular markers based upon whole chloroplast genomes and identifying alpine Gentiana waltonii and G. lhassica (Gentianaceae)
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Bo-ran DONG1, Zhi-li ZHAO1, *, Liang-hong NI1, Dorje GAAWE2, Tong-hua LIU2, 3
Acta Pharmaceutica Sinica | 2021, 56(9) : 2584 - 2591
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Acta Pharmaceutica Sinica | 2021, 56(9): 2584-2591
• Original Articles •
Molecular markers based upon whole chloroplast genomes and identifying alpine Gentiana waltonii and G. lhassica (Gentianaceae)
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Bo-ran DONG1, Zhi-li ZHAO1, *, Liang-hong NI1, Dorje GAAWE2, Tong-hua LIU2, 3
Affiliations
  • 1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. Tibetan Traditional Medical College, Lhasa 850000, China
  • 3. Beijing University of Chinese Medicine, Beijing 100029, China
Published: 2021-09-12 doi: 10.16438/j.0513-4870.2021-0527
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As two original plants of Tibetan herb Jieji, Gentiana waltonii Burk. and Gentiana lhassica Burk. belong to Section Cruciata of Gentiana, Gentianaceae. Here, we report on whole chloroplast genome sequences in the alpine species, respectively, and the features of plastomes were investigated. The plastome of G. waltonii is 148 705 bp long (148 652 bp in G. lhassica) and encodes 112 genes, including 78 protein-coding genes, 30 transfer RNA genes, and 4 ribosomal RNA genes. Two pseudogenes, namely ψrps16 and ψinfA, were found in plastomes. In addition, two novel loci were detected, and a species-specific polymerase chain reaction assay was developed for differentiating G. waltonii and G. lhassica from 10 alpine species in Section Cruciata. Gentiana. Our study provides basic data for identifying Tibetan herbs, alpine species conservation and molecular phylogenetic studies of Gentiana and Gentianaceae.

Tibetan herb  /  Gentiana waltonii  /  Gentiana lhassica  /  plastome  /  molecular loci  /  species-specific PCR assay
Bo-ran DONG, Zhi-li ZHAO, Liang-hong NI, Dorje GAAWE, Tong-hua LIU. Molecular markers based upon whole chloroplast genomes and identifying alpine Gentiana waltonii and G. lhassica (Gentianaceae)[J]. Acta Pharmaceutica Sinica, 2021 , 56 (9) : 2584 -2591 . DOI: 10.16438/j.0513-4870.2021-0527
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Year 2021 volume 56 Issue 9
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Article Info
doi: 10.16438/j.0513-4870.2021-0527
  • Receive Date：2021-04-12
  • Online Date：2025-12-18
  • Published：2021-09-12
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  • Received：2021-04-12
  • Revised：2021-05-06
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Affiliations
    1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Tibetan Traditional Medical College, Lhasa 850000, China
    3. Beijing University of Chinese Medicine, Beijing 100029, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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