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Identification of Gastrodiae Rhizoma chromatographic peaks identification by liner calibration with two reference substances assisted
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Ya-nan ZHOU1, Yuan-yuan ZHANG1, Hui-jun LI2, Yong-li LIU1, *, Lei SUN3, Shuang-cheng MA3, *
Acta Pharmaceutica Sinica | 2021, 56(7) : 1797 - 1803
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Acta Pharmaceutica Sinica | 2021, 56(7): 1797-1803
• Special Reports: Focalizing on the research of the quality control and in vivo process of natural drugs •
Identification of Gastrodiae Rhizoma chromatographic peaks identification by liner calibration with two reference substances assisted
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Ya-nan ZHOU1, Yuan-yuan ZHANG1, Hui-jun LI2, Yong-li LIU1, *, Lei SUN3, Shuang-cheng MA3, *
Affiliations
  • 1. Hebei Institute for Drug and Medical Device Control, Shijiazhuang 050227, China
  • 2. China Pharmaceutical University of Traditional Chinese Pharmacy, Nanjing 210009, China
  • 3. National Institutes for Food and Drug Control, Beijing 100050, China
Published: 2021-07-12 doi: 10.16438/j.0513-4870.2021-0526
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This study aimed to establish a method for positioning six chromatographic peaks occurred in HPLC profile of Gastrodiae Rhizoma. The "liner calibration with two reference substances" (LCTRS) method was used to calculate the retention time so as to assist in positioning of chromatographic peaks in terms of the prediction accuracy of retention time and the coincidence rate of chromatographic column. A total of 24 C18 chromatographic columns from different brands and types available were used to determine the retention times of six components in Gastrodiae Rhizoma, then the average retention time of each component was obtained as standard retention time (SRT). Parishin E (peak 3) and Parishin A (peak 6) were simultaneously taken as reference substance to forecast the retention time of the other four components by using the LCTRS method. Four different C18 columns were employed to verify the method. Meanwhile, for the purpose of comparison, the relative retention time (RRT) method was applied to forecast the retention time, by using Parishin E as the single reference substance. The comparison between LCTRS and RRT methods indicated that the former was more accurate in predicting the retention time and more applicable in utilization of chromatographic columns. This study demonstrated that the LCTRS method shows the superior performance in positioning of chromatographic peak, and therefore has a good prospect of application.

Gastrodiae Rhizoma  /  liner calibration with two reference substances  /  relative retention time  /  chromatographic peak positioning
Ya-nan ZHOU, Yuan-yuan ZHANG, Hui-jun LI, Yong-li LIU, Lei SUN, Shuang-cheng MA. Identification of Gastrodiae Rhizoma chromatographic peaks identification by liner calibration with two reference substances assisted[J]. Acta Pharmaceutica Sinica, 2021 , 56 (7) : 1797 -1803 . DOI: 10.16438/j.0513-4870.2021-0526
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Year 2021 volume 56 Issue 7
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Article Info
doi: 10.16438/j.0513-4870.2021-0526
  • Receive Date：2021-04-14
  • Online Date：2025-12-18
  • Published：2021-07-12
Article Data
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History
  • Received：2021-04-14
  • Revised：2021-06-24
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Affiliations
    1. Hebei Institute for Drug and Medical Device Control, Shijiazhuang 050227, China
    2. China Pharmaceutical University of Traditional Chinese Pharmacy, Nanjing 210009, China
    3. National Institutes for Food and Drug Control, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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