Home Journals Articles Updates About
CN
image
收藏切换
Expression profiling of glycosaminoglycans in the skin of type 2 diabetic mice based on liquid chromatography-mass spectrometry
收藏切换
PDF
Lang DING1, Xin ZHANG1, Qin-ying LI1, Chen WANG1, Qing-sen SHANG1, 2, Chao CAI1, 2, Guo-yun LI1, 2, *, Guang-li YU1, 2, *
Acta Pharmaceutica Sinica | 2021, 56(10) : 2841 - 2848
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(10): 2841-2848
• Original Articles •
Expression profiling of glycosaminoglycans in the skin of type 2 diabetic mice based on liquid chromatography-mass spectrometry
Full
Lang DING1, Xin ZHANG1, Qin-ying LI1, Chen WANG1, Qing-sen SHANG1, 2, Chao CAI1, 2, Guo-yun LI1, 2, *, Guang-li YU1, 2, *
Affiliations
  • 1. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • 2. Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Published: 2021-10-12 doi: 10.16438/j.0513-4870.2021-0476
Outline
收藏切换

Type 2 diabetes is a common form of diabetes and can have serious consequences for diabetics when their wounds do not heal properly due to impaired function. Glycosaminoglycans (GAGs) are widely found in skin tissue. Due to the isomerization of C6 in uronic acid and different sulfuric acid substitutions, the structure of GAGs is relatively complex. The fine structure of GAGs in the skin of diabetic patients has not been studied. In this study, the structure of GAGs in the skin of streptozotocin (STZ)-induced diabetic mice was characterized by liquid chromatographytandem mass spectrometry (LC-MS/MS). All animal experiments were carried out with approval of the Animal Ethics Committee of Ocean University of China. The results indicate that the content of hyaluronic acid (HA) in STZ-induced diabetic mouse skin was significantly lower than that of non-diabetic mice. Although there was no significant difference in the content of chondroitin sulfate (CS) and heparin sulfate (HS) between diabetic mouse and normal mouse skin, the disaccharide compositions were different. The expression of CS-4S6S, HS-0S, HS-NS, and HS-6S in STZ-induced diabetic mouse skin was higher than in non-diabetic mice. While the content of HS-NS6S was lower. In addition, the degree of sulfation of HS in STZ-induced mouse skin was lower than that of normal mouse skin. These results provide a basis for the pathogenesis of diabetes and the development of wound healing dressings for diabetic patients.

diabetes  /  glycosaminoglycan  /  liquid chromatography-mass spectrometry  /  skin
Lang DING, Xin ZHANG, Qin-ying LI, Chen WANG, Qing-sen SHANG, Chao CAI, Guo-yun LI, Guang-li YU. Expression profiling of glycosaminoglycans in the skin of type 2 diabetic mice based on liquid chromatography-mass spectrometry[J]. Acta Pharmaceutica Sinica, 2021 , 56 (10) : 2841 -2848 . DOI: 10.16438/j.0513-4870.2021-0476
Appendix
Less
Year 2021 volume 56 Issue 10
PDF
155
60
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-0476
  • Receive Date：2021-04-01
  • Online Date：2025-12-18
  • Published：2021-10-12
Article Data
Affiliations
History
  • Received：2021-04-01
  • Revised：2021-05-10
Funding
Affiliations
    1. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
    2. Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-0476
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House