Home Journals Articles Updates About
CN
image
收藏切换
Simultaneous determination of seven components in Citri Exocarpium Rubrum by UPLC-MS/MS
收藏切换
PDF
Shuai-shuai FAN1, Hai-bo REN1, Meng-ting YANG1, Xin-guo WANG1, 2, 3, 4, Li-ying NIU1, 2, 3, 4, *
Acta Pharmaceutica Sinica | 2021, 56(10) : 2835 - 2840
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(10): 2835-2840
• Original Articles •
Simultaneous determination of seven components in Citri Exocarpium Rubrum by UPLC-MS/MS
Full
Shuai-shuai FAN1, Hai-bo REN1, Meng-ting YANG1, Xin-guo WANG1, 2, 3, 4, Li-ying NIU1, 2, 3, 4, *
Affiliations
  • 1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China
  • 2. Hebei TCM Formula Granule Innovation Center, Shijiazhuang 050091, China
  • 3. Hebei TCM Quality Evaluation and Standardization Engineering Research Center, Shijiazhuang 050091, China
  • 4. TCM Formula Granule Research Center of Hebei Province University, Shijiazhuang 050091, China
Published: 2021-10-12 doi: 10.16438/j.0513-4870.2021-0460
Outline
收藏切换

An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of seven components (chlorogenic acid, rutin, narirutin, hesperidin, sinensetin, nobiletin, tangeretin) in Citri Exocarpium Rubrum. The separation was performed over 12.0 min on a Shim-pack GIST C18 column (100 mm×2.1 mm, 2.0 μm) with a mobile phase consisting of acetonitrile (A) and 0.1% formic acid water solution (B) with gradient elution at a flow rate of 0.4 mL·min-1; the column temperature was 30℃ and the injection volume was 1 μL. Detection was performed on a QTRAP 4500 mass spectrometer equipped with electrospray ionization. Positive and negative electrospray ionization was performed in multiple reaction monitoring mode. All of the analytes showed good linearity (r ≥ 0.997 3) in the tested ranges, with good precision, repeatability and stability for all analytes. The average recoveries were in the range of 99.32%-107.75% with relative standard deviations RSD ≤ 3.94%. The established method is accurate, stable and reproducible, and can provide a research method for the quality control of Citri Exocarpium Rubrum.

Citri Exocarpium Rubrum  /  UPLC-MS/MS  /  content determination  /  quality control
Shuai-shuai FAN, Hai-bo REN, Meng-ting YANG, Xin-guo WANG, Li-ying NIU. Simultaneous determination of seven components in Citri Exocarpium Rubrum by UPLC-MS/MS[J]. Acta Pharmaceutica Sinica, 2021 , 56 (10) : 2835 -2840 . DOI: 10.16438/j.0513-4870.2021-0460
Appendix
Less
Year 2021 volume 56 Issue 10
PDF
126
53
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-0460
  • Receive Date：2021-03-30
  • Online Date：2025-12-18
  • Published：2021-10-12
Article Data
Affiliations
History
  • Received：2021-03-30
  • Revised：2021-05-17
Funding
Affiliations
    1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China
    2. Hebei TCM Formula Granule Innovation Center, Shijiazhuang 050091, China
    3. Hebei TCM Quality Evaluation and Standardization Engineering Research Center, Shijiazhuang 050091, China
    4. TCM Formula Granule Research Center of Hebei Province University, Shijiazhuang 050091, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-0460
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House