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Ophiopogonin D interferes with ferroptosis to reduce the damage of cardiomyocytes induced by ophiopogonin D'
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Yi LIN1, Chun-qi YANG1, 2, Wen-yu LIAN1, 3, Cheng-rong XIAO1, Hong-ling TAN1, Yue GAO1, *, Yu-guang WANG1, *
Acta Pharmaceutica Sinica | 2021, 56(8) : 2241 - 2247
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Acta Pharmaceutica Sinica | 2021, 56(8): 2241-2247
• Original Articles •
Ophiopogonin D interferes with ferroptosis to reduce the damage of cardiomyocytes induced by ophiopogonin D'
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Yi LIN1, Chun-qi YANG1, 2, Wen-yu LIAN1, 3, Cheng-rong XIAO1, Hong-ling TAN1, Yue GAO1, *, Yu-guang WANG1, *
Affiliations
  • 1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
  • 2. Anhui Medical University, Hefei 230032, China
  • 3. Guangdong Pharmaceutical University, Guangzhou 510006, China
Published: 2021-08-12 doi: 10.16438/j.0513-4870.2021-0419
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This study investigated the intervention effect and possible mechanism of ophiopogonin D (OPD) in protecting cardiomyocytes against ophiopogonin D' (OPD')-induced injury, and provided relevant experimental data for the clinical use of Ophiopogon japonicas. Cell counting kit-8 (CCK-8) assay was used to evaluate the effect of OPD and OPD' on H9c2 cell viability. The content of reaction oxygen species (ROS) in cells were detected by flow cytometry. The contents of Fe2+ in cells were detected by FerroOrange's fluorescence imaging. The content of glutathione (GSH) and glutathione peroxidase (GSH-Px) were detected by kits. The expression of transferrin receptor 1 (TFR1), cyclooxygenase 2 (COX2), NADPH oxidase 1 (NOX1), long-chain acyl-CoA synthetase 4 (ACSL4), cationic amino acid transporter 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and ferritin heavy chain 1 (FTH1) was detected by Western blot. Results showed that OPD' (1 μmol·L-1) significantly induced the expression of ferroptosis-related proteins, the contents of Fe2+, ROS, and GSH-Px were increased, and the content of GSH were decreased. In addition, different concentrations of OPD (0.5, 1, and 2 μmol·L-1) could partially reverse the myocardial cell injury caused by OPD', and the best effect was obtained when the dose range was 1-2 μmol·L-1. The experimental results show that OPD can interfere with the ferroptosis caused by OPD', and then have a protective effect on H9c2 cells.

Ophiopogon japonicas  /  ophiopogonin D  /  ophiopogonin D'  /  cardiomyocyte  /  ferroptosis
Yi LIN, Chun-qi YANG, Wen-yu LIAN, Cheng-rong XIAO, Hong-ling TAN, Yue GAO, Yu-guang WANG. Ophiopogonin D interferes with ferroptosis to reduce the damage of cardiomyocytes induced by ophiopogonin D'[J]. Acta Pharmaceutica Sinica, 2021 , 56 (8) : 2241 -2247 . DOI: 10.16438/j.0513-4870.2021-0419
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Article Info
doi: 10.16438/j.0513-4870.2021-0419
  • Receive Date：2021-03-24
  • Online Date：2025-12-18
  • Published：2021-08-12
Article Data
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History
  • Received：2021-03-24
  • Revised：2021-04-30
Funding
Affiliations
    1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China
    2. Anhui Medical University, Hefei 230032, China
    3. Guangdong Pharmaceutical University, Guangzhou 510006, China
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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