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Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution
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Wen YANG1, Hai-yan YU1, Dong WANG2, Xue-mei ZHOU2, Jian-ping ZHANG2, Lei GAO3, *
Acta Pharmaceutica Sinica | 2021, 56(11) : 3112 - 3117
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Acta Pharmaceutica Sinica | 2021, 56(11): 3112-3117
• Original Articles •
Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution
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Wen YANG1, Hai-yan YU1, Dong WANG2, Xue-mei ZHOU2, Jian-ping ZHANG2, Lei GAO3, *
Affiliations
  • 1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010000, China
  • 2. Inner Mongolia Key Laboratory of Chinese and Mongolian Medicines Standard Research, Inner Mongolia Pharmaceutical Inspection Institute, Hohhot 010000, China
  • 3. Inner Mongolia Pharmacovigilance Center, Hohhot 010000, China
Published: 2021-11-12 doi: 10.16438/j.0513-4870.2021-0266
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A simple and efficient method for extracting Cistanche polysaccharides was obtained and used to extract four Cistanche polysaccharides. The molecular weight, distribution and polysaccharide mass fraction of the four Cistanche polysaccharides were measured by High performance size exclusion chromatography-multiangle laser light scatter-refractive index detector (HPSEC-MALLS-RID) and differences between the four Cistanche polysaccharides were determined. The results of a single factor test and an orthogonal test showed that the best process parameters for extracting Cistanche polysaccharides used a mixture of enzyme (4%), a ratio of complex enzyme (cellulase∶pectinase) 1∶1, ultrasonic power at 350 W (40 kHz), for 20 min, enzymolysis temperature of 50 ℃, and pH 6. The average yield of three repeated extractions was 5.14%, the mass fraction was 90.78%, the comprehensive index was 47.96%, and the RSD = 1.51%, indicating good reproducibility. The four Bossica Cistanche polysaccharides are all spherical structures but their molecular weights and distributions differ. The optimized compounded enzyme combined with ultrasonic extraction increases the yield of Cistanche polysaccharides. It is a simple, convenient, economical and environmentally friendly extraction method, which lays the foundation for future polysaccharide research.

Cistanche deserticola polysaccharide  /  complex enzyme  /  molecular weight
Wen YANG, Hai-yan YU, Dong WANG, Xue-mei ZHOU, Jian-ping ZHANG, Lei GAO. Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution[J]. Acta Pharmaceutica Sinica, 2021 , 56 (11) : 3112 -3117 . DOI: 10.16438/j.0513-4870.2021-0266
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Article Info
doi: 10.16438/j.0513-4870.2021-0266
  • Receive Date：2021-06-23
  • Online Date：2025-12-18
  • Published：2021-11-12
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History
  • Received：2021-06-23
  • Revised：2021-09-15
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Affiliations
    1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010000, China
    2. Inner Mongolia Key Laboratory of Chinese and Mongolian Medicines Standard Research, Inner Mongolia Pharmaceutical Inspection Institute, Hohhot 010000, China
    3. Inner Mongolia Pharmacovigilance Center, Hohhot 010000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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