Home Journals Articles Updates About
CN
image
收藏切换
Identification of Fritillaria taibaiensis and its relatives by real-time PCR with a TaqMan-MGB probe
收藏切换
PDF
Tian ZHANG1, Jiao CHEN1, Rui-ping JIANG1, Meng ZOU1, Tie-chui YANG2, Shao-bing FU2, Jia-yu ZHOU1, *, Hai LIAO1, *
Acta Pharmaceutica Sinica | 2021, 56(9) : 2577 - 2583
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(9): 2577-2583
• Original Articles •
Identification of Fritillaria taibaiensis and its relatives by real-time PCR with a TaqMan-MGB probe
Full
Tian ZHANG1, Jiao CHEN1, Rui-ping JIANG1, Meng ZOU1, Tie-chui YANG2, Shao-bing FU2, Jia-yu ZHOU1, *, Hai LIAO1, *
Affiliations
  • 1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
  • 2. Qinghai Lvkang Biological Development Co., Ltd., Xining 810003, China
Published: 2021-09-12 doi: 10.16438/j.0513-4870.2021-0230
Outline
收藏切换

The molecular identification of Fritillaria taibaiensis and its relatives was studied by real-time PCR with a TaqMan-MGB probe. DNA was extracted from F. taibaiensis and its relatives. According to the sequence of ITS1 region, the mutation sites of F. taipaiensis and its related species were identified by MEGA7.0 software. The specific primers (a pair) and a TaqMan-MGB probe were designed by Primer Premier 6.0 software. In the Roche LightCycler 96 system, the lowest limit of detection for F. taipaiensis DNA template was 0.002 39 ng·μL-1, and the optimal Tm value range was 60 and 61℃. Specificity identification showed that the method had good specificity for F. taipaiensis, as it could be distinguished from other 13 different Fritillaria species including F. unibracteata. Since this method could accurately identify F. taipaiensis and its related species, it provides technical support for rational development of F. taipaiensis resources, management of Chinese medicinal market and supervision of raw materials in Chinese medicine manufacturing enterprises.

TaqMan-MGB probe  /  Fritillaria taipaiensis  /  ITS1  /  real-time PCR  /  molecular identification
Tian ZHANG, Jiao CHEN, Rui-ping JIANG, Meng ZOU, Tie-chui YANG, Shao-bing FU, Jia-yu ZHOU, Hai LIAO. Identification of Fritillaria taibaiensis and its relatives by real-time PCR with a TaqMan-MGB probe[J]. Acta Pharmaceutica Sinica, 2021 , 56 (9) : 2577 -2583 . DOI: 10.16438/j.0513-4870.2021-0230
Appendix
Less
Year 2021 volume 56 Issue 9
PDF
219
90
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-0230
  • Receive Date：2021-02-14
  • Online Date：2025-12-18
  • Published：2021-09-12
Article Data
Affiliations
History
  • Received：2021-02-14
  • Revised：2021-03-15
Funding
Affiliations
    1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
    2. Qinghai Lvkang Biological Development Co., Ltd., Xining 810003, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-0230
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House