Home Journals Articles Updates About
CN
image
收藏切换
DNA barcoding identification of commercial decoctions in traditional Chinese medicine
收藏切换
PDF
Tian-yi XIN1, Qian LOU1, Li-jun HAO1, Ran-jun LI1, 2, Yu-lin LIN1, Wen-jie XU1, Ting-yu MA1, Ye-xuan LIN3, Yi-xin DONG3, Cheng-yu JIANG3, Jing-yuan SONG1, 4, *
Acta Pharmaceutica Sinica | 2021, 56(3) : 879 - 889
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(3): 879-889
• Original Articles •
DNA barcoding identification of commercial decoctions in traditional Chinese medicine
Full
Tian-yi XIN1, Qian LOU1, Li-jun HAO1, Ran-jun LI1, 2, Yu-lin LIN1, Wen-jie XU1, Ting-yu MA1, Ye-xuan LIN3, Yi-xin DONG3, Cheng-yu JIANG3, Jing-yuan SONG1, 4, *
Affiliations
  • 1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • 2. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
  • 3. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Biochemistry, Peking Union Medical College, Beijing 100005, China
  • 4. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China
Published: 2021-03-12 doi: 10.16438/j.0513-4870.2020-1750
Outline
收藏切换

Although the guiding principles for molecular identification of traditional Chinese medicines (TCM) using DNA barcoding have been recorded in the Chinese Pharmacopoeia, there is still a lack of systematic research on its application to commercial TCM decoctions. In this study, a total of 212 commercial TCM decoctions derived from different medicinal parts such as root and rhizome, fruit and seed, herb, flower, leaf, cortex, and caulis were collected to verify applicability and accuracy of the method. DNA barcodes were successfully obtained from 75.9% (161/212) of the samples, while other samples failed to be amplified due to genomic DNA degradation. Among the 161 samples, 85.7% of them were identified as recorded species in the Chinese Pharmacopoeia (2020 edition). In addition, 14 samples could be identified as species recorded in the Chinese Pharmacopoeia and their closely related species in the same genus. Morphological identification for the unconfirmed samples showed that eight were genuine species and three were adulterants, while the other three were unidentifiable due to lack of morphological characteristics. Furthermore, the DNA barcodes of seven samples accurately mapped to the sequences of adulterants. Remarkably, counterfeit products were detected in two samples. These results demonstrate that DNA barcoding is suitable for the identification of commercial TCM decoctions. The method can effectively detect adulterants and is appropriate for use throughout the industrial chain of TCM production and distribution, and by the supervisory agencies as well.

decoction pieces  /  DNA barcoding  /  ITS2  /  psbA-trnH  /  molecular identification  /  market supervision
Tian-yi XIN, Qian LOU, Li-jun HAO, Ran-jun LI, Yu-lin LIN, Wen-jie XU, Ting-yu MA, Ye-xuan LIN, Yi-xin DONG, Cheng-yu JIANG, Jing-yuan SONG. DNA barcoding identification of commercial decoctions in traditional Chinese medicine[J]. Acta Pharmaceutica Sinica, 2021 , 56 (3) : 879 -889 . DOI: 10.16438/j.0513-4870.2020-1750
Appendix
Less
Year 2021 volume 56 Issue 3
PDF
179
68
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2020-1750
  • Receive Date：2020-11-17
  • Online Date：2025-12-18
  • Published：2021-03-12
Article Data
Affiliations
History
  • Received：2020-11-17
  • Revised：2020-12-04
Funding
Affiliations
    1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
    2. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
    3. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Department of Biochemistry, Peking Union Medical College, Beijing 100005, China
    4. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2020-1750
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House