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The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology
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Yuan HE, Qi-rui MU, Zhen-fang BAI, Dan JIANG, Guang-xi REN, Chun-sheng LIU*
Acta Pharmaceutica Sinica | 2021, 56(3) : 872 - 878
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Acta Pharmaceutica Sinica | 2021, 56(3): 872-878
Original Articles
The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology
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Yuan HE, Qi-rui MU, Zhen-fang BAI, Dan JIANG, Guang-xi REN, Chun-sheng LIU*
Affiliations
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
Published: 2021-03-12 doi: 10.16438/j.0513-4870.2020-1627
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In order to provide a scientific basis for the establishment of a Daphnes Cortex medicinal material fungus library and the screening of endophytic fungi that promote the growth of Daphnes Cortex and increase the content of daphnetin, we used Illumina high-throughput testing technology to analyze 9 Daphnes Cortex samples from Gansu and Shanxi provinces. A total of 632 766 valid sequences were obtained, including 348 OTUs, 4 phyla, 20 classes, 48 orders, 108 families, 154 genera, and 208 species. The sum of the first 3 fungal genera account for more than 65% of the total abundance, with the highest reaching 98.4%. Alternaria and Phoma are the main genuses of Daphne giraldii Nitsche, and Altemaria is the dominant genus. The endophytic fungi community of Daphnes Cortex is rich in diversity, and the order of fungal diversity in different producing areas is: Gangu County > Wutai County > Tanchang County.

high-throughput sequencing  /  Daphnes Cortex  /  endophytic fungi  /  diversity  /  endangered plant
Yuan HE, Qi-rui MU, Zhen-fang BAI, Dan JIANG, Guang-xi REN, Chun-sheng LIU. The diversity of endophytic fungi in endangered medicinal material Daphnes Cortex as determined by high-throughput sequencing technology[J]. Acta Pharmaceutica Sinica, 2021 , 56 (3) : 872 -878 . DOI: 10.16438/j.0513-4870.2020-1627
Year 2021 volume 56 Issue 3
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Article Info
doi: 10.16438/j.0513-4870.2020-1627
  • Receive Date:2020-10-19
  • Online Date:2025-12-18
  • Published:2021-03-12
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  • Received:2020-10-19
  • Revised:2020-12-07
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    School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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