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Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation
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Zhi-quan WEI1, Chuan-hong BAO2, Yi-xin CHEN2, Li YAN1, *
Acta Pharmaceutica Sinica | 2021, 56(6) : 1644 - 1652
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Acta Pharmaceutica Sinica | 2021, 56(6): 1644-1652
• Original Articles •
Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation
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Zhi-quan WEI1, Chuan-hong BAO2, Yi-xin CHEN2, Li YAN1, *
Affiliations
  • 1. Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions, GuangxiTraditional Chinese Medicine University, Nanning 530001, China
  • 2. Guangxi Key Laboratory of Efficacy Studyon Chinese Materia Medica, Guangxi Traditional Chinese Medicine University, Nanning 530200, China
Published: 2021-06-12 doi: 10.16438/j.0513-4870.2020-1623
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In this study, in vitro experiments were conducted to investigate that sinomenine inhibits the macrophage classic activation by up-regulating the expression of paired immunoglobulin-like receptor B (PIR-B). A macrophage model with classic activation was established by lipopolysaccharide and interferon-gamma co-stimulation. Real-time fluorescence reverse transcription-polymerase chain reaction was executed for evaluating the PIR-B gene expression, and Western blot for PIR-B protein expression, in macrophages, respectively. The tumor necrosis factor α and interleukin 8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay. The flow cytometry was utilized to detect M1 macrophages. The PIR-B expression in situ was observed by laser scanning confocal microscope. The results showed that sinomenine significantly increased the expression of PIR-B, markedly reduced the percentage of M1 macrophages, and decreased the levels of tumor necrosis factor α and interleukin 8 in the culture supernatant. The above results indicated that sinomenine can significantly inhibit the macrophage classic activation, and its mechanism may be related to the increase of PIR-B expression in macrophages. This pharmacological effect helps explain the pharmacodynamic mechanism of sinomenine in treating rheumatoid arthritis.

sinomenine  /  rheumatoid arthritis  /  paired immunoglobulin-like receptor B  /  macrophage classic activation  /  M1 macrophage
Zhi-quan WEI, Chuan-hong BAO, Yi-xin CHEN, Li YAN. Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation[J]. Acta Pharmaceutica Sinica, 2021 , 56 (6) : 1644 -1652 . DOI: 10.16438/j.0513-4870.2020-1623
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Article Info
doi: 10.16438/j.0513-4870.2020-1623
  • Receive Date：2020-10-19
  • Online Date：2025-12-18
  • Published：2021-06-12
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History
  • Received：2020-10-19
  • Revised：2021-02-16
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Affiliations
    1. Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions, GuangxiTraditional Chinese Medicine University, Nanning 530001, China
    2. Guangxi Key Laboratory of Efficacy Studyon Chinese Materia Medica, Guangxi Traditional Chinese Medicine University, Nanning 530200, China
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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