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Anti-dengue virus activity of natural podocarpane-type diterpenoid in vitro
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Fang WANG1, 2, Huan YAN3, Zhai-wen YAO1, 4, Rong-hua LUO1, Hai-yang LIU3, *, Yong-tang ZHENG1, *
Acta Pharmaceutica Sinica | 2021, 56(3) : 793 - 798
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Acta Pharmaceutica Sinica | 2021, 56(3): 793-798
• Original Articles •
Anti-dengue virus activity of natural podocarpane-type diterpenoid in vitro
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Fang WANG1, 2, Huan YAN3, Zhai-wen YAO1, 4, Rong-hua LUO1, Hai-yang LIU3, *, Yong-tang ZHENG1, *
Affiliations
  • 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
  • 2. College of Medicine, Kunming University of Science and Technology, Kunming 650500, China
  • 3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany Chinese Academy of Sciences, Kunming 650201, China
  • 4. College of Pharmaceutical Sciences, Soochow University, Suzhou 215021, China
Published: 2021-03-12 doi: 10.16438/j.0513-4870.2020-1451
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Dengue virus (DENV) is the most rapidly transmitted mosquito-borne pathogen, which is the main cause of seasonal outbreaks of dengue fever and dengue hemorrhagic fever in tropical and subtropical regions, and may cause serious life-threatening diseases. There is an urgent need to develop effective vaccines or antiviral therapies. In this paper, we found that a podocarpane-type diterpenoid, (3α, 5β, 10α)-13-methoxypodocarpa-8, 11, 13-triene-3, 12-diol (MPTD), isolated from the stems and leaves of Aleurites moluccana, showed good effect against DENV. The anti-DENV activity of MPTD against four different DENV serotypes was studied by plaque assay. The cytotoxicity of MPTD in Vero and Huh7 cells was tested by MTT assay. qRT-PCR and Western blot assays were used to investigate the anti-DENV activity of MPTD at RNA and protein levels, respectively. The results showed that MPTD greatly reduced the virus titer in DENV infected Vero cells, and its 50% effective concentration (EC50) for DENV (1-4) were 2.72±0.39, 10.99±5.18, 18.72±0.21, and 0.48±0.28 μmol·L-1, respectively. The results showed that MPTD inhibits DENV RNA level and the expression of E protein. In addition, MPTD may inhibit the early stage of DENV replication and exert antiviral activity. Further studies showed that the inhibitory effect of MPTD against DENV infection is not targeting the viral entry stage. Therefore, MPTD has a significant anti-dengue virus effect, and is an anti-DENV compound with potential application value.

Aleurites moluccana  /  natural compound  /  (3α, 5β, 10α)-13-methoxypodocarpa-8, 11, 13-triene-3, 12-diol  /  podocarpane-type diterpenoid  /  dengue virus  /  antiviral activity
Fang WANG, Huan YAN, Zhai-wen YAO, Rong-hua LUO, Hai-yang LIU, Yong-tang ZHENG. Anti-dengue virus activity of natural podocarpane-type diterpenoid in vitro[J]. Acta Pharmaceutica Sinica, 2021 , 56 (3) : 793 -798 . DOI: 10.16438/j.0513-4870.2020-1451
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Article Info
doi: 10.16438/j.0513-4870.2020-1451
  • Receive Date：2020-09-04
  • Online Date：2025-12-18
  • Published：2021-03-12
Article Data
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History
  • Received：2020-09-04
  • Revised：2020-10-27
Funding
Affiliations
    1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
    2. College of Medicine, Kunming University of Science and Technology, Kunming 650500, China
    3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany Chinese Academy of Sciences, Kunming 650201, China
    4. College of Pharmaceutical Sciences, Soochow University, Suzhou 215021, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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