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Cloning and bioinformatic analysis of the 3-ketoacyl-CoA synthase gene in Coix lacryma-jobi L.
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Xiao-yan WEI1, 2, Yong LI2, Juan GUO2, Ya-nan WANG*, 2, Lu-qi HUANG*, 1, 2
Acta Pharmaceutica Sinica | 2021, 56(2) : 610 - 617
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Acta Pharmaceutica Sinica | 2021, 56(2): 610-617
• Original Articles •
Cloning and bioinformatic analysis of the 3-ketoacyl-CoA synthase gene in Coix lacryma-jobi L.
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Xiao-yan WEI1, 2, Yong LI2, Juan GUO2, Ya-nan WANG*, 2, Lu-qi HUANG*, 1, 2
Affiliations
  • 1. Academician Workstation, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • 2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Published: 2021-02-12 doi: 10.16438/j.0513-4870.2020-1407
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As a key gene in the regulation of long-chain fatty acid biosynthesis, 3-ketoacyl-CoA synthase (KCS) plays an important role in the growth and development of Coix lacryma-jobi L. In this study, the KCS gene was cloned from cDNA of Coix lachryma-Jobi L. and bioinformatics analysis was performed. Results showed that the full length KCS gene was 1 548 bp encoding 515 amino acids. Bioinformatics analysis indicated that the gene encoded a 58 608.12 Da protein with an isoelectric point of 9.20 containing two transmembrane helical structure domains and lacking a signal peptide, with a likely subcellular localization in main plastid membranes. The results of multiple sequence comparisons and evolutionary tree analysis revealed that KCS had three identical conserved sequences and was closely related to KCS from monocotyledons such as Sorghum bicolor, Zea mays, Setaria italica, Panicum miliaceum, Oryza brachyantha, Hordeum vulgare, Aegilops tauschii subsp. Tauschii. We speculated that the evolution of the gene was similar among these plants of the same family. In addition, gene expression analysis showed that the KCS gene was significantly different in Coix lacryma-jobi L. isolates having different lipid content. This work will facilitate further study of the regulatory mechanism of this enzyme in fatty acid synthesis.

Coix lacryma-jobi L.  /  3-ketoacyl-CoA synthase  /  gene cloning  /  bioinformatics analysis
Xiao-yan WEI, Yong LI, Juan GUO, Ya-nan WANG, Lu-qi HUANG. Cloning and bioinformatic analysis of the 3-ketoacyl-CoA synthase gene in Coix lacryma-jobi L.[J]. Acta Pharmaceutica Sinica, 2021 , 56 (2) : 610 -617 . DOI: 10.16438/j.0513-4870.2020-1407
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Year 2021 volume 56 Issue 2
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Article Info
doi: 10.16438/j.0513-4870.2020-1407
  • Receive Date：2020-08-26
  • Online Date：2025-12-18
  • Published：2021-02-12
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History
  • Received：2020-08-26
  • Revised：2020-09-27
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Affiliations
    1. Academician Workstation, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
    2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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