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We investigated the ability of 3-bromopyruvic acid to increase the sensitivity of tamoxifen-resistant MCF-7/TR cells to tamoxifen and to explore the underlying mechanism. 4-Hydroxy tamoxifen (4-OHT) is the active form of tamoxifen in vivo and was used in this study. The effect of 3-bromopyruvic acid on the viability of MCF-7/TR cells was measured by MTT assay and the morphology of MCF-7/TR cells was observed with an inverted microscope. The results show that 3-bromopyruvic acid at 40 μmol·L-1 has no significant effect on the viability of MCF-7/TR cells, and this concentration was used in the subsequent experiments:3-bromopyruvic acid significantly increased the inhibitory effect of 4-OHT on the viability of MCF-7/TR cells, with a reversal-fold of 1.91, as determined by MTT assay; the lactate level, determined with a lactate detection kit, and the expression levels of GLUT1, HK2, and LDHA in MCF-7/TR cells as measured by immunoblotting were significantly higher than in MCF-7 cells; and compared with 4-OHT treatment alone, the combination treatment of 3-bromopyruvic acid and 4-OHT significantly reduced the lactate level and the expression of GLUT1, HK2, LDHA in MCF-7/TR cells. The above results show that 3-bromopyruvic acid increases the sensitivity of MCF-7/TR cells to tamoxifen, and the mechanism is related to the inhibition of aerobic glycolysis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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