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Use of ITS2 and psbA-trnH DNA barcode sequences of wild Phytolacca resources in Guanzhong area of Shaanxi Province
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Rui-hua Lü1, Zhao FENG1, Tian-yi MA1, Rui-hua Lü1, Ming-ying ZHANG2, Liang PENG2, Jing GAO2, Gang ZHANG2, *
Acta Pharmaceutica Sinica | 2020, 55(8) : 1951 - 1956
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Acta Pharmaceutica Sinica | 2020, 55(8): 1951-1956
• Original Articles •
Use of ITS2 and psbA-trnH DNA barcode sequences of wild Phytolacca resources in Guanzhong area of Shaanxi Province
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Rui-hua Lü1, Zhao FENG1, Tian-yi MA1, Rui-hua Lü1, Ming-ying ZHANG2, Liang PENG2, Jing GAO2, Gang ZHANG2, *
Affiliations
  • 1. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
  • 2. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
Published: 2020-08-12 doi: 10.16438/j.0513-4870.2020-0278
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In order to explore the use of DNA barcode in the identification of wild Phytolacca resources in the Shaanxi Guanzhong area, 29 DNA samples were amplified and sequenced by using the universal primers ITS2 and psbA-trnH. The sequences were spliced and proof-read by Codon CodeA aligner V3.0, followed by blast comparison and identification analysis; mega 6.0 was used to analyze sequence characteristics, Kimura 2-Parameter (K2P) was used to analyze distance and intraspecific or interspecific variation, and Neighbor-Joining trees were established to evaluate the ability of two pairs of candidate sequences to distinguish Phytolaccae Radix from its adulterants. The results showed that the success rate of PCR amplification and sequencing of ITS2 and psbA-trnH was 100%; the NJ tree showed that both ITS2 and psbA-trnH sequences could separate P. acinosa, P. americana, other species of the same genus like P. japonica, P. exiensis and two adulterant species into a single clade; primer ITS2 had an advantage over psbA-trnH in determining interspecific genetic distances. Therefore, both ITS2 and psbA-trnH sequences can be used for identification of Phytolacca and their adulterants, which provides a theoretical basis for the distribution of wild Phytolacca resources and their rational development and utilization.

Phytolacca acinosa Roxb.  /  DNA barcode  /  ITS2  /  psbA-trnH  /  molecular identification
Rui-hua Lü, Zhao FENG, Tian-yi MA, Rui-hua Lü, Ming-ying ZHANG, Liang PENG, Jing GAO, Gang ZHANG. Use of ITS2 and psbA-trnH DNA barcode sequences of wild Phytolacca resources in Guanzhong area of Shaanxi Province[J]. Acta Pharmaceutica Sinica, 2020 , 55 (8) : 1951 -1956 . DOI: 10.16438/j.0513-4870.2020-0278
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Year 2020 volume 55 Issue 8
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Article Info
doi: 10.16438/j.0513-4870.2020-0278
  • Receive Date：2020-03-10
  • Online Date：2026-01-21
  • Published：2020-08-12
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  • Received：2020-03-10
  • Revised：2020-04-02
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Affiliations
    1. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
    2. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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