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Advances in chromatography-based methods for screening active compounds from natural products
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Jing-yi JIAN1, Hui-huang CHEN2, Qi-sheng HONG2, Lü-huan WANG1, Yu-mei ZHAO1, Lei LI1, Ting-ting ZHANG1, Hai-bo ZHOU1, Zheng-jin JIANG1, *
Acta Pharmaceutica Sinica | 2020, 55(7) : 1504 - 1510
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Acta Pharmaceutica Sinica | 2020, 55(7): 1504-1510
• Reviews •
Advances in chromatography-based methods for screening active compounds from natural products
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Jing-yi JIAN1, Hui-huang CHEN2, Qi-sheng HONG2, Lü-huan WANG1, Yu-mei ZHAO1, Lei LI1, Ting-ting ZHANG1, Hai-bo ZHOU1, Zheng-jin JIANG1, *
Affiliations
  • 1. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China
  • 2. Rehabilitation Department of the First Affiliated Hospital of Jinan University, Guangzhou 510630, China
Published: 2020-07-12 doi: 10.16438/j.0513-4870.2020-0088
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Natural products have been a major source of leading compounds in drug discovery. How to effectively screen active compounds from complex matrix remains an interesting topic. In this review, we comprehensively summarized advanced liquid chromatography based approaches in natural products screening, including pre-column, on-column and post-column screening methods. Their advantages, disadvantages and prospect are also discussed.

natural product  /  active compounds screening  /  ultrafiltration  /  magnetic beads based ligand fishing  /  cellular membrane chromatography  /  cellular membrane affinity chromatography  /  immobilized enzyme reactor  /  on-line bioassay-guided screening  /  bioassay-guided fractionation
Jing-yi JIAN, Hui-huang CHEN, Qi-sheng HONG, Lü-huan WANG, Yu-mei ZHAO, Lei LI, Ting-ting ZHANG, Hai-bo ZHOU, Zheng-jin JIANG. Advances in chromatography-based methods for screening active compounds from natural products[J]. Acta Pharmaceutica Sinica, 2020 , 55 (7) : 1504 -1510 . DOI: 10.16438/j.0513-4870.2020-0088
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Year 2020 volume 55 Issue 7
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Article Info
doi: 10.16438/j.0513-4870.2020-0088
  • Receive Date：2020-02-05
  • Online Date：2026-01-21
  • Published：2020-07-12
Article Data
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History
  • Received：2020-02-05
  • Revised：2020-05-05
Funding
Affiliations
    1. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China
    2. Rehabilitation Department of the First Affiliated Hospital of Jinan University, Guangzhou 510630, China
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多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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