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Exploring the molecular mechanism of aqueous extract from Huang qi on doxorubicin induced nephropathy in rats using transcriptome sequencing technique
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Sheng-sheng HE1, 2, Ai-ping LI1, *, Wang-ning ZHANG1, Xue-mei QIN1, *
Acta Pharmaceutica Sinica | 2019, 54(8) : 1439 - 1448
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Acta Pharmaceutica Sinica | 2019, 54(8): 1439-1448
• Original Articles •
Exploring the molecular mechanism of aqueous extract from Huang qi on doxorubicin induced nephropathy in rats using transcriptome sequencing technique
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Sheng-sheng HE1, 2, Ai-ping LI1, *, Wang-ning ZHANG1, Xue-mei QIN1, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
Published: 2019-08-12 doi: 10.16438/j.0513-4870.2019-0225
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We studied the effect of aqueous extract from Huang qi on gene expression profile of doxorubicin induced nephropathy in rats, and explored the molecular mechanism of the intervention. The gene expression profiles of control group, model group and aqueous extract from Huang qi group were detected by using transcriptome sequencing technique. The differentially expressed genes (DEGs) were screened by STEM trend analysis software. GO function enrichment and KEGG pathway analysis were performed for DEGs, and the gene expression level was verified by real-time fluorescence quantitative PCR (RT-qPCR). The results showed that, compared with the control group, 432 DEGs were obtained in doxorubicin nephropathy model group; compared with the model group, 811 DEGs were obtained due to aqueous extract of Huang qi. The results of GO function enrichment and KEGG enrichment analysis indicated that PI3K-AKT pathway (Col6a6, Nr4a1, Sgk1, Gng7) and lipid metabolism-related genes (Cpt1b, Pcsk9, Abca1, Ascm5) were the key pathways and genes in the treatment of doxorubicin induced nephropathy by aqueous extract from Huang qi, which played a protective role in kidney. In conclusion, the molecular mechanism of aqueous extract from Huang qi in protection against doxorubicin induced nephropathy rats is closely related to apoptosis-related genes and lipid metabolism-related genes, suggesting for the need of follow-up study for key gene validation and mechanism of action of aqueous extract from Huang qi for prevention of doxorubicin induced nephropathy.

Huang qi  /  aqueous extract  /  doxorubicin nephropathy  /  transcriptomics  /  molecular mechanism
Sheng-sheng HE, Ai-ping LI, Wang-ning ZHANG, Xue-mei QIN. Exploring the molecular mechanism of aqueous extract from Huang qi on doxorubicin induced nephropathy in rats using transcriptome sequencing technique[J]. Acta Pharmaceutica Sinica, 2019 , 54 (8) : 1439 -1448 . DOI: 10.16438/j.0513-4870.2019-0225
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doi: 10.16438/j.0513-4870.2019-0225
  • Receive Date：2019-04-01
  • Online Date：2026-01-26
  • Published：2019-08-12
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  • Received：2019-04-01
  • Revised：2019-06-04
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    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
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Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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