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Measurement of antiplatelet aggregation and potency of hirudo
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Xuan WANG1, 2, Qi-chao GAN3, Jing-yan SHI3, Huang-wan-yin HU2, Jun-jie HAO2, Guo-song WU2, Qian GAO1, Xiang LI1, Ming NIU2, *, Jia-bo WANG2, *, Li MA1, *, Xiao-he XIAO2
Acta Pharmaceutica Sinica | 2019, 54(12) : 2178 - 2183
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Acta Pharmaceutica Sinica | 2019, 54(12): 2178-2183
• Special Reports: Biological Evaluation and Precise Control for the Quality of Traditional Chinese Medicines •
Measurement of antiplatelet aggregation and potency of hirudo
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Xuan WANG1, 2, Qi-chao GAN3, Jing-yan SHI3, Huang-wan-yin HU2, Jun-jie HAO2, Guo-song WU2, Qian GAO1, Xiang LI1, Ming NIU2, *, Jia-bo WANG2, *, Li MA1, *, Xiao-he XIAO2
Affiliations
  • 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • 2. China Military Institute of Chinese Medicine, Integrative Medical Center, Fifth Medical Center, General Hospital of Chinese PLA, Beijing 100039, China
  • 3. Chongqing Duoputai Pharmaceutical Co., Ltd., Chongqing 401420, China
Published: 2019-12-12 doi: 10.16438/j.0513-4870.2019-0147
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The objective of this work was to develop a bioassay to quantify the antiplatelet aggregation activity of hirudo for quality evaluation and control. Antithrombin activity of hirudo extracted by high temperature decoction was determined by thrombin titration. Antiplatelet aggregation activity of hirudo was determined through pharmacodynamic experiments in vitro and in vivo using a bioassay we developed for quantifying inhibition of platelet aggregation. Methodological investigation was carried out and the titers of 12 batches of hirudo samples were determined. During the experiment, the disposal of animals is in accordance with the ethical standards of animal experiments. The results showed that the antithrombin activity of hirudo decocted at high temperature decreased significantly and almost lost its activity. Hirudo inhibited platelet aggregation and results in vivo and in vitro were consistent. These assays were employed to test 12 batches of hirudo. The results demonstrated that the biopotency of 12 batches was 113.49, 96.13, 121.22, 127.33, 83.48, 108.72, 131.41, 127.95, 76.90, 126.27, 132.89 and 573.53 U·mg-1. The method was reliable and reproducible and can be used to assess the quality of hirudo.

hirudo  /  promoting blood circulation  /  anti-platelet aggregation  /  potency  /  quality evaluation
Xuan WANG, Qi-chao GAN, Jing-yan SHI, Huang-wan-yin HU, Jun-jie HAO, Guo-song WU, Qian GAO, Xiang LI, Ming NIU, Jia-bo WANG, Li MA, Xiao-he XIAO. Measurement of antiplatelet aggregation and potency of hirudo[J]. Acta Pharmaceutica Sinica, 2019 , 54 (12) : 2178 -2183 . DOI: 10.16438/j.0513-4870.2019-0147
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Year 2019 volume 54 Issue 12
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Article Info
doi: 10.16438/j.0513-4870.2019-0147
  • Receive Date：2019-03-02
  • Online Date：2026-01-26
  • Published：2019-12-12
Article Data
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History
  • Received：2019-03-02
  • Revised：2019-04-26
Funding
Affiliations
    1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    2. China Military Institute of Chinese Medicine, Integrative Medical Center, Fifth Medical Center, General Hospital of Chinese PLA, Beijing 100039, China
    3. Chongqing Duoputai Pharmaceutical Co., Ltd., Chongqing 401420, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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