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Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins
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Qi-han XIA1, Ming GUO1, 2, *, Ping HOU1, Yi GU1, Fu-rong ZHAO1
Acta Pharmaceutica Sinica | 2019, 54(5) : 906 - 912
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Acta Pharmaceutica Sinica | 2019, 54(5): 906-912
• Original Articles •
Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins
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Qi-han XIA1, Ming GUO1, 2, *, Ping HOU1, Yi GU1, Fu-rong ZHAO1
Affiliations
  • 1. School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
  • 2. School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
Published: 2019-05-12 doi: 10.16438/j.0513-4870.2018-1146
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The method for analyzing the interaction between caffeine and human serum albumin (HSA) was established by capillary electrophoresis. Under physiological conditions, the interaction between ligand (caffeine)-receptor (HSA) was studied with frontier-analysis (FA) method, Hummel-Dreyer (HD) method and plug-plug kinetic (PPK) method. The interaction parameters of caffeine-HSA system were obtained using non-linear equation, Scatchard equation and Klotz equation. The results showed that FA, HD and PPK methods were suitable for caffeine-HSA system, among them, HD method was the best, and the Non-linear equation was the best theoretical model to caffeine-HSA system. Interaction parameter tests showed that caffeine-HSA interaction was a single site interaction and the binding stability was moderate. The mechanism of caffeine-HSA interaction has been elucidated, which can provide valuable information for further research of alkaloids.

alkaloid  /  caffeine  /  human serum albumin  /  theoretical model  /  interaction parameter
Qi-han XIA, Ming GUO, Ping HOU, Yi GU, Fu-rong ZHAO. Comparison and theoretical modeling of capillary electrophoresis analysis for the interaction between caffeine and serum proteins[J]. Acta Pharmaceutica Sinica, 2019 , 54 (5) : 906 -912 . DOI: 10.16438/j.0513-4870.2018-1146
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Article Info
doi: 10.16438/j.0513-4870.2018-1146
  • Receive Date：2018-12-25
  • Online Date：2026-01-26
  • Published：2019-05-12
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  • Received：2018-12-25
  • Revised：2019-02-01
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    1. School of Forestry and Bio-technology, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
    2. School of Science, Zhejiang Agricultural & Forestry University, Hangzhou 311300, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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