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Rapid and simultaneous determination of nifedipine and dehydronifedipine in human plasma
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Xue-fei CHEN1, 2, Xiang-yu HOU2, Xiao-yan CHEN2, Da-fang ZHONG1, 2, *
Acta Pharmaceutica Sinica | 2019, 54(2) : 360 - 365
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Acta Pharmaceutica Sinica | 2019, 54(2): 360-365
• Original Articles •
Rapid and simultaneous determination of nifedipine and dehydronifedipine in human plasma
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Xue-fei CHEN1, 2, Xiang-yu HOU2, Xiao-yan CHEN2, Da-fang ZHONG1, 2, *
Affiliations
  • 1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China
  • 2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
Published: 2019-02-12 doi: 10.16438/j.0513-4870.2018-0764
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Nifedipine, a calcium channel antagonist, is metabolized mainly by CYP3A4 to dehydronifedipine. A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine nifedipine and dehydronifedipine in human plasma using d6-nifedipine/d6-dehydronifedipine as internal standards. After extraction from the plasma by protein precipitation, the analytes and internal standard were separated on a Hypersil Gold C18 (50 mm×2.1 mm, 1.9 μm). The mobile phase consisted of methanol and 5 mmol·L-1ammonium acetate aqueous solution (0.1% formic acid). Positive electrospray ionization was performed using multiple reaction monitoring (MRM) with transitions of m/z 347.3→254.1 for nifedipine, m/z 345.2→283.9 for dehydronifedipine, m/z 353.3→257.1 for d6-nifedipine, m/z 351.2→286.9 for d6-dehydronifedipine. The method had a linear calibration curves over the concentrations of 0.10-80.0 ng·mL-1 for nifedipine and 0.050-40.0 ng·mL-1 for dehydronifedipine. The validated LC-MS/MS method has been successfully used study pharmacokinetic interactions of apatinib (CYP3A4 inhibitor) and nifedipine (CYP3A4 substrate) in human. This clinical trial was approved by the society of ethics and conducted in the first hospital of China medical university.

LC-MS/MS  /  nifedipine  /  dehydronifedipine
Xue-fei CHEN, Xiang-yu HOU, Xiao-yan CHEN, Da-fang ZHONG. Rapid and simultaneous determination of nifedipine and dehydronifedipine in human plasma[J]. Acta Pharmaceutica Sinica, 2019 , 54 (2) : 360 -365 . DOI: 10.16438/j.0513-4870.2018-0764
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Article Info
doi: 10.16438/j.0513-4870.2018-0764
  • Receive Date：2018-08-20
  • Online Date：2026-01-26
  • Published：2019-02-12
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History
  • Received：2018-08-20
  • Revised：2018-10-15
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    1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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