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Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
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Ye-ting ZOU1, 2, Jin-di XU2, Fang LONG1, 3, Ye-qing ZHANG3, Song-lin LI1, 2, *
Acta Pharmaceutica Sinica | 2019, 54(1) : 130 - 137
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Acta Pharmaceutica Sinica | 2019, 54(1): 130-137
• ORIGINAL ARTICLES •
Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode
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Ye-ting ZOU1, 2, Jin-di XU2, Fang LONG1, 3, Ye-qing ZHANG3, Song-lin LI1, 2, *
Affiliations
  • 1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
  • 2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
  • 3. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
Published: 2019-01-12 doi: 10.16438/j.0513-4870.2018-0670
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An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method was developed to evaluate the chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof. First, high resolution mass spectrometry data were obtained with Full scan mode, by comparing with MS data from the reference compounds and literatures, a total of 23 components were unequivocally or tentatively identified in ethanol extracts and acetic ether extracts thereof. Then, a mimic multiple reaction monitoring (mMRM) mode was established using UPLC-QTOF-MS/MS to quantify the triterpene acids in ethanol extracts and acetic ether extracts thereof. Eleven components were absolutely quantified with reference compounds, while 12 components without reference compounds were relatively quantified with peak areas, the transfer and enrichment rate of triterpene acids during liquid-liquid extraction were calculated. It was found all of the 23 triterpene acids identified in Poria ethanol extracts could be transferred into acetic ether extracts with high transfer and enrichment rate. The present study provides not only scientific evidence for further extraction of triterpene acids in Poria by acetic ether, but also an approach for comprehensive evaluation of the chemical consistency of herbal medicine extracts before and after the liquid-liquid extraction.

Poria  /  triterpene acid  /  liquid-liquid extraction  /  chemical consistency  /  UPLC-QTOF-MS/MS
Ye-ting ZOU, Jin-di XU, Fang LONG, Ye-qing ZHANG, Song-lin LI. Evaluation of chemical consistency of triterpene acids in ethanol extracts of Poria and acetic ether extracts thereof by UPLC-QTOF-MS/MS with full scan and mimic MRM mode[J]. Acta Pharmaceutica Sinica, 2019 , 54 (1) : 130 -137 . DOI: 10.16438/j.0513-4870.2018-0670
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Article Info
doi: 10.16438/j.0513-4870.2018-0670
  • Receive Date：2018-07-23
  • Online Date：2026-01-26
  • Published：2019-01-12
Article Data
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History
  • Received：2018-07-23
  • Revised：2018-09-19
Funding
Affiliations
    1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
    2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of Chinese Academy of Traditional Chinese Medicine, Nanjing 210028, China
    3. Department of Respiratory Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine to Nanjing University of Chinese Medicine, Nanjing 210028, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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