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Studies on the enzymatic synthesis of a new PPT-type ginsenoside via UDP-glycosyltransferase PgUGT74AE2 from Panax ginseng
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Ting-ting ZHANG, Hui-chao LIANG, Ting GONG, Zong-feng HU, An-di GU, Jin-ling YANG*, Ping ZHU*
Acta Pharmaceutica Sinica | 2018, 53(9) : 1565 - 1570
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Acta Pharmaceutica Sinica | 2018, 53(9): 1565-1570
• ORIGINAL ARTICLES •
Studies on the enzymatic synthesis of a new PPT-type ginsenoside via UDP-glycosyltransferase PgUGT74AE2 from Panax ginseng
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Ting-ting ZHANG, Hui-chao LIANG, Ting GONG, Zong-feng HU, An-di GU, Jin-ling YANG*, Ping ZHU*
Affiliations
  • State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products of National Health Commission of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Published: 2018-09-12 doi: 10.16438/j.0513-4870.2018-0300
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UDP-glycosyltransferase PgUGT74AE2 from Panax ginseng can transfer a glucose moiety to the free C-3 hydroxyl of protopanaxadiol (PPD) to produce ginsenoside Rh2. However, no report demonstrates that PgUGT74AE2 can transfer a glucose moiety to the free C-3 hydroxyl of protopanaxatriol (PPT) to produce a PPT-type ginsenoside. In this study, the expression plasmid pET-32a-PgUGT74AE2 was constructed for expression of the recombinant protein and transferred into Escherichia coli Transetta (DE3) to generate the recombinant strain Transetta-PgUGT74AE2. The recombinant enzyme PgUGT74AE2 was expressed by induction of isopropyl-β-D-thiogalactoside (IPTG). An in vitro enzymatic reaction system was established with the recombinant enzyme PgUGT74AE2 and the substrate PPT. PgUGT74AE2 catalyzed the glycosylation of the free C-3 hydroxyl of PPT to produce 3-O-β-D-glucopyranosyl-dammar-24-ene-3β, 6α, 12β, 20S-tetraol, a new PPT-type ginsenoside. This study provides an efficient approach for the biosynthesis of a new PPT-type ginsenoside through in vitro enzymatic reaction, which may pave a way to produce promising lead in drug discovery.

Panax ginseng  /  glycosyltransferase  /  glycosylation  /  protopanaxatriol  /  protopanaxatriol-type ginsenoside
Ting-ting ZHANG, Hui-chao LIANG, Ting GONG, Zong-feng HU, An-di GU, Jin-ling YANG, Ping ZHU. Studies on the enzymatic synthesis of a new PPT-type ginsenoside via UDP-glycosyltransferase PgUGT74AE2 from Panax ginseng[J]. Acta Pharmaceutica Sinica, 2018 , 53 (9) : 1565 -1570 . DOI: 10.16438/j.0513-4870.2018-0300
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Year 2018 volume 53 Issue 9
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Article Info
doi: 10.16438/j.0513-4870.2018-0300
  • Receive Date：2018-04-04
  • Online Date：2026-01-15
  • Published：2018-09-12
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  • Received：2018-04-04
  • Revised：2018-04-27
Funding
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    State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products of National Health Commission of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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