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Effect of triptolide on the expression and function of P-gp in nasopharyngeal cancer cells
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Xiu WANG1, Na ZHU1, Jian-chun LI1, Chuan-bin WU1, 2, Qing-qing WANG1, *
Acta Pharmaceutica Sinica | 2018, 53(7) : 1107 - 1112
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Acta Pharmaceutica Sinica | 2018, 53(7): 1107-1112
• ORIGINAL ARTICLES·Pharmacology •
Effect of triptolide on the expression and function of P-gp in nasopharyngeal cancer cells
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Xiu WANG1, Na ZHU1, Jian-chun LI1, Chuan-bin WU1, 2, Qing-qing WANG1, *
Affiliations
  • 1. School of Pharmacy, Bengbu Medical College, Bengbu 233030, China
  • 2. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
Published: 2018-07-12 doi: 10.16438/j.0513-4870.2018-0064
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This study is designed to investigate the effect of triptolide on the function and expression of P-glycoprotein (P-gp) in HNE1 nasopharyngeal cancer cells. MTT assay was used to test cell viability. Intracellular doxorubicin content was evaluated with flow cytometry. Rhodamine 123 (Rh) was used to detect the excretion function of P-gp. The expression of P-gp was analyzed by Western blot. ATP levels were evaluated. JC-1 staining was used to determining mitochondrial membrane potential (MMP). Triptolide, doxorubicin and the combination treatment all had the inhibitory effect to HNE1 cells, and the combination treatment had the best effect. Triptolide increased intracellular concentration of doxorubicin and Rh (P < 0.05 or P < 0.01), inhibited the excretion function of P-gp. The expression of P-gp was reduced greatly in the middle and high dose group of triptolide. The ATP levels were decreased significantly (P < 0.05). JC-1 staining showed that triptolide mediated the down-regulation of MMP in HNE1 cells. Triptolide could increase intracellular drug content and enhance cytotoxicity of chemotherapeutics by inhibition of the expression and the excretion function of P-gp.

triptolide  /  nasopharyngeal cancer  /  P-glycoprotein  /  drug resistance
Xiu WANG, Na ZHU, Jian-chun LI, Chuan-bin WU, Qing-qing WANG. Effect of triptolide on the expression and function of P-gp in nasopharyngeal cancer cells[J]. Acta Pharmaceutica Sinica, 2018 , 53 (7) : 1107 -1112 . DOI: 10.16438/j.0513-4870.2018-0064
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doi: 10.16438/j.0513-4870.2018-0064
  • Receive Date：2018-01-16
  • Online Date：2026-01-15
  • Published：2018-07-12
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  • Received：2018-01-16
  • Revised：2018-02-05
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    1. School of Pharmacy, Bengbu Medical College, Bengbu 233030, China
    2. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
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红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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