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Construction and screening of phage display library for TIM-3 nanobody
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Lin-lin MA1, Min ZHU2, Guang-hui LI2, Yan-fei LI1, Jun-wei GAI2, Ya-kun WAN2, *
Acta Pharmaceutica Sinica | 2018, 53(3) : 388 - 395
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Acta Pharmaceutica Sinica | 2018, 53(3): 388-395
• ORIGINAL ARTICLES·Pharmacology •
Construction and screening of phage display library for TIM-3 nanobody
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Lin-lin MA1, Min ZHU2, Guang-hui LI2, Yan-fei LI1, Jun-wei GAI2, Ya-kun WAN2, *
Affiliations
  • 1. Key Laboratory of Molecular Imaging of Shanghai Education Commission, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
  • 2. Shanghai Novamab Biopharmaceuticals Co., Ltd., Shanghai 201318, China
Published: 2018-03-12 doi: 10.16438/j.0513-4870.2017-1092
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Immunotherapy is a new strategy for cancer treatment that has the potential to treat all types of cancer. T cell immunoglobulin and mucin-domain-containing molecule-3 (TIM-3) is a key negative regulator of T cell activation. TIM-3 blockage using anti-TIM-3 monoclonal antibody therapy has a great appeal and special advantages. Nanobodies, derived from heavy chain fragment in camelid animals, are now proving clinical values in the development of antibody drugs. In this study, we have immunized camel with TIM-3 antigens and then constructed phage display library. Moreover, 29 nanobodies with different complementarity-determining regions sequences have been screened from the phage display library by phage display technology. In addition, we successfully constructed the cell line stably expressing TIM-3, and screened 10 TIM-3 nanobodies with high specificity and high affinity using flow cytometry. Our study will lay the foundation for the future screening and development of anti-TIM-3 whole humanized functional nanobody.

T cell immunoglobulin and mucin-domain-containing molecule-3  /  nanobody  /  phage display library
Lin-lin MA, Min ZHU, Guang-hui LI, Yan-fei LI, Jun-wei GAI, Ya-kun WAN. Construction and screening of phage display library for TIM-3 nanobody[J]. Acta Pharmaceutica Sinica, 2018 , 53 (3) : 388 -395 . DOI: 10.16438/j.0513-4870.2017-1092
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Year 2018 volume 53 Issue 3
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Article Info
doi: 10.16438/j.0513-4870.2017-1092
  • Receive Date：2017-11-16
  • Online Date：2026-01-15
  • Published：2018-03-12
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History
  • Received：2017-11-16
  • Revised：2017-12-12
Funding
Affiliations
    1. Key Laboratory of Molecular Imaging of Shanghai Education Commission, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
    2. Shanghai Novamab Biopharmaceuticals Co., Ltd., Shanghai 201318, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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