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Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes
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Li-li HUANG2, Ke ZAN1, Li-nong GUO1, Jie LIU1, Jian ZHENG1, *, Shuang-cheng MA1, 2, *
Acta Pharmaceutica Sinica | 2018, 53(4) : 598 - 603
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Acta Pharmaceutica Sinica | 2018, 53(4): 598-603
• ORIGINAL ARTICLES·Pharmaceutical Analysis •
Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes
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Li-li HUANG2, Ke ZAN1, Li-nong GUO1, Jie LIU1, Jian ZHENG1, *, Shuang-cheng MA1, 2, *
Affiliations
  • 1. National Institutes for Food and Drug Control, Beijing 100050, China
  • 2. Guangxi Institute for Food and Drug Control, Nanning 530021, China
Published: 2018-04-12 doi: 10.16438/j.0513-4870.2017-0940
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This study was designed to establish a method to obtain the fingerprint chromatogram for the quantitative determination of Cordyceps sinensis in different sizes, a comparison of Cordyceps sinensis from five places was made to analyze its similarity and the content of main nucleosides (uridine, inoside, guanosine and adenosine). The assay was performed on a Waters XSelect HSS T3 C18 (4.6 mm×250 mm, 5 μm), with a mobile phase consisting of water (A)-acetonitrile (B) at the flow rate of 0.6 mL·min-1 (0-5 min, 0 B; 5-15 min, 0→10% B; 15-30 min, 10%→20% B; 30-33 min, 20%→50% B; 33-35 min, 50%→0 B; 35-40 min, 0 B). The detection wavelength was 260 nm and the column temperature was set at 30℃, and the injection volume was 5 μL. The results showed that there was no significant difference of the nucleosides in samples from the same place of the different sizes, but contents of the nucleosides variate a lot by production places. More data are required for further research. The method is proved for scientific and specific formulation of the standard in evaluation of circulated Cordyceps sinensis.

Cordyceps sinensis  /  different sizes  /  fingerprint chromatogram  /  nucleosides
Li-li HUANG, Ke ZAN, Li-nong GUO, Jie LIU, Jian ZHENG, Shuang-cheng MA. Comparative study on nucleosides in circulated Cordyceps sinensis of different sizes[J]. Acta Pharmaceutica Sinica, 2018 , 53 (4) : 598 -603 . DOI: 10.16438/j.0513-4870.2017-0940
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doi: 10.16438/j.0513-4870.2017-0940
  • Receive Date：2017-10-21
  • Online Date：2026-01-15
  • Published：2018-04-12
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  • Received：2017-10-21
  • Revised：2017-12-20
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    1. National Institutes for Food and Drug Control, Beijing 100050, China
    2. Guangxi Institute for Food and Drug Control, Nanning 530021, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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