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Analysis of metabolites and metabolic pathway of 5F-AMB in vitro using UPLC-HR-MS
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Jing LI*, Zhen-dong HUA, You-mei WANG
Acta Pharmaceutica Sinica | 2017, 52(11) : 1743 - 1747
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Acta Pharmaceutica Sinica | 2017, 52(11): 1743-1747
• ORIGINAL ARTICLES •
Analysis of metabolites and metabolic pathway of 5F-AMB in vitro using UPLC-HR-MS
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Jing LI*, Zhen-dong HUA, You-mei WANG
Affiliations
  • National Narcotic Laboratory, Drug Intelligence and Forensic Center of Minister of Public Security, Beijing 100193, China
Published: 2017-11-12 doi: 10.16438/j.0513-4870.2017-0670
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This study was designed to investigate the metabolites of 5F-AMB by human liver microsomes model in vitro. 5F-AMB was added in the reaction mixture to simulate the metabolic process in human hepatocytes in vivo, and then to determine the reaction points and pathways of metabolism by ultra performance liquid chromatography (UPLC) coupled to high resolution mass spectrum (HR-MS). 5F-AMB generated 9 metabolites in total in the human liver microsomes model. Ester hydrolysis, combination of ester hydrolysis and oxidative defluorination, combination of ester hydrolysis and hydroxylation on pentyl chain moiety and combination of ester hydrolysis and hydroxylation on indazole ring moiety reactions were its main metabolic pathways. The method is fast and efficient so that the ester hydrolysis, combination of ester hydrolysis and oxidative defluorination, combination of ester hydrolysis and hydroxylation on pentyl chain moiety and combination of ester hydrolysis and hydroxylation on indazole ring moiety metabolites of 5F-AMB can be used as the suitable and potential biomarkers in the urine samples.

methyl (2S)-2-{[1-(5-fluoropentyl)-1H-indazol-3-yl]formamido}-3-methylbutanoate  /  synthetic cannabinoids  /  high resolution mass spectrum  /  human liver microsomes  /  in vitro
Jing LI, Zhen-dong HUA, You-mei WANG. Analysis of metabolites and metabolic pathway of 5F-AMB in vitro using UPLC-HR-MS[J]. Acta Pharmaceutica Sinica, 2017 , 52 (11) : 1743 -1747 . DOI: 10.16438/j.0513-4870.2017-0670
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Article Info
doi: 10.16438/j.0513-4870.2017-0670
  • Receive Date：2017-07-09
  • Online Date：2026-01-14
  • Published：2017-11-12
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  • Received：2017-07-09
  • Revised：2017-08-08
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    National Narcotic Laboratory, Drug Intelligence and Forensic Center of Minister of Public Security, Beijing 100193, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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