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Identification of Dendrobium huoshanense, Dendrobium officinale and Dendrobium devonianum by multiplex allele-specific polymerase chain reaction
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Yu-qin LUO1, 3, Chao JIANG2, Yuan YUAN2, *, Su-ping XIAO3, Run-huai ZHAO3, *, Ge LI4, Yu-yang ZHAO2
Acta Pharmaceutica Sinica | 2017, 52(6) : 998 - 1006
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Acta Pharmaceutica Sinica | 2017, 52(6): 998-1006
• ORIGINAL ARTICLES·Pharmacognosy •
Identification of Dendrobium huoshanense, Dendrobium officinale and Dendrobium devonianum by multiplex allele-specific polymerase chain reaction
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Yu-qin LUO1, 3, Chao JIANG2, Yuan YUAN2, *, Su-ping XIAO3, Run-huai ZHAO3, *, Ge LI4, Yu-yang ZHAO2
Affiliations
  • 1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • 2. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • 3. Chinese Traditional Medicine Company of China, Beijing 100195, China
  • 4. Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China
Published: 2017-06-12 doi: 10.16438/j.0513-4870.2016-1207
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This study was designed to establish a multiplex allele-specific polymerase chain reaction method for simultaneous identification of Dendrobium huoshanense, D. officinale and D. devonianum, which may resolve identification problems of caulis dendrobii. Internal transcribed spacer sequences and trnL-trnF sequences of the Dendrobium species were aligned by BioEdit software, then specific SNPs of the three species were analyzed for designing allele-specific primers and the multiplex allele-specific PCR reaction system was established. The different origin of Dendrobium huoshanense, D. officinale and D. devonianum was amplified and identified by the sizes of respective band. The results showed that 584 bp, 397 bp and 211 bp bands could be amplified by D. devonianum, Dendrobium officinale and Dendrobium huoshanense respectively, when the annealing temperature was 61 ℃ and the number of cycles was 35. The limit of detection (LOD) of D. devonianum and D. huoshanense were both 1.2 ng, while D. officinale was low than 0.24 ng. The detection limit of adulterates in D. devonianum, D. devonianum and D. huoshanense mixture sample was 1%, 1% and 5% respectively. This result suggests that the method of multiplex allele-specific PCR is useful to identify D. huoshanense, D. officinale and D. devonianum is accurate and specific.

Dendrobium huoshanense  /  Dendrobium officinale  /  Dendrobium devonianum  /  multiplex allele-specific polymerase chain reaction  /  admixture identification
Yu-qin LUO, Chao JIANG, Yuan YUAN, Su-ping XIAO, Run-huai ZHAO, Ge LI, Yu-yang ZHAO. Identification of Dendrobium huoshanense, Dendrobium officinale and Dendrobium devonianum by multiplex allele-specific polymerase chain reaction[J]. Acta Pharmaceutica Sinica, 2017 , 52 (6) : 998 -1006 . DOI: 10.16438/j.0513-4870.2016-1207
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Article Info
doi: 10.16438/j.0513-4870.2016-1207
  • Receive Date：2016-12-22
  • Online Date：2026-01-14
  • Published：2017-06-12
Article Data
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History
  • Received：2016-12-22
  • Revised：2017-01-15
Funding
Affiliations
    1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
    2. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
    3. Chinese Traditional Medicine Company of China, Beijing 100195, China
    4. Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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