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Rapid identification of chemical constituents in Huangqi Jianzhong Tang by UHPLC coupled with hybrid quadrupole-orbitrap MS
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Ying-huan HU1, 2, 3, Wen-qian XU1, 2, 3, Xue-mei QIN1, 3, *, Yue-tao LIU1, 3, *
Acta Pharmaceutica Sinica | 2017, 52(6) : 964 - 970
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Acta Pharmaceutica Sinica | 2017, 52(6): 964-970
• ORIGINAL ARTICLES·Pharmaceutical Analysis •
Rapid identification of chemical constituents in Huangqi Jianzhong Tang by UHPLC coupled with hybrid quadrupole-orbitrap MS
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Ying-huan HU1, 2, 3, Wen-qian XU1, 2, 3, Xue-mei QIN1, 3, *, Yue-tao LIU1, 3, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
  • 3. Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan 030006, China
Published: 2017-06-12 doi: 10.16438/j.0513-4870.2016-1191
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This study was designed to establish an ultra-liquid chromatography-mass spectrometry method for the reliable identification the multiple chemical components in Huangqi Jianzhong Tang (HQJZ). The ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry method was applied to identify the chemical constituents in the HQJZ rapidly. A total of 71 compounds including two major categories of saponins and flavonoids were identified or tentatively deduced on the basis of their retention behaviors, fragments of multistage mass spectrometry or by comparing with reference substances and literatures. Among them, 20 compounds were from Astragali Radix, 14 were from Paeoniae Radix Alba, 37 were from Glycyrrhizae Radix et Rhizoma Praeparata cum Melle, 3 were from Rhizoma Zingiberis Recens, 2 were from Cinnamomi Ramulus and Jujubae Fructus, respectively. The LC-MS method was used to qualitatively analyze the chemical constituents of HQJZ, which provides a scientific basis for the quality control of HQJZ.

Huangqi Jianzhong Tang  /  chemical constituent  /  UHPLC-Q Exactive quadrupole-orbitrap MS
Ying-huan HU, Wen-qian XU, Xue-mei QIN, Yue-tao LIU. Rapid identification of chemical constituents in Huangqi Jianzhong Tang by UHPLC coupled with hybrid quadrupole-orbitrap MS[J]. Acta Pharmaceutica Sinica, 2017 , 52 (6) : 964 -970 . DOI: 10.16438/j.0513-4870.2016-1191
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doi: 10.16438/j.0513-4870.2016-1191
  • Receive Date：2016-12-30
  • Online Date：2026-01-14
  • Published：2017-06-12
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  • Received：2016-12-30
  • Revised：2017-02-04
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Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
    3. Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan 030006, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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