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Identification of water buffalo horn and its adulterants using COI barcode
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Xu-zhao LIU1, 2, Li-si ZHOU1, Jin-xin LIU1, 3, Jing JIA1, Jing-yuan SONG1, *, Lin-chun SHI1, *
Acta Pharmaceutica Sinica | 2017, 52(3) : 494 - 499
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Acta Pharmaceutica Sinica | 2017, 52(3): 494-499
Identification of water buffalo horn and its adulterants using COI barcode
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Xu-zhao LIU1, 2, Li-si ZHOU1, Jin-xin LIU1, 3, Jing JIA1, Jing-yuan SONG1, *, Lin-chun SHI1, *
Affiliations
  • 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • 2. College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • 3. Hebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical College, Chengde 067000, China
Published: 2017-03-12 doi: 10.16438/j.0513-4870.2016-1020
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Bubali cornu (water buffalo horn) has been used as the substitute for Cornu rhinoceri asiatici (rhino horn) in clinical applications, and is the essential ingredient of Angong Niuhuang Wan. In recent years, there are a number of adulterants on the commercial herbal medicine markets. An efficient tool is required for species identification. In this study, 155 Bubali cornu samples have been taken from original animals and collected from commercial herbal medicine markets. 153 COI sequences have been successfully obtained from 155 samples through DNA extraction, PCR amplification, bidirectional sequencing and assembly. 93 COI sequences have been added to the DNA barcoding database of traditional Chinese animal medicine after validation using DNA barcoding GAP and tree-based methods. The species identification of the 62 commercial Bubali cornu medicines has been accomplished on the DNA barcoding system for identifying herbal medicine using the updated animal medicine database (www.tcmbarcode.cn). Except two samples failed to obtain COI sequences, 54.8% of the commercial Bubali cornu medicines were water buffalo horns and 29% were yak horns. Our results showed that yak horn was the major adulterant of Bubali cornu and the DNA barcoding method may accurately discriminate Bubali cornu and their adulterants. Therefore, we recommend that supervision on the herbal medicine markets should be strengthened with this new method to warren the effectiveness of herbal medicines.

water buffalo horn  /  COI sequences  /  DNA barcoding  /  identification  /  adulterants
Xu-zhao LIU, Li-si ZHOU, Jin-xin LIU, Jing JIA, Jing-yuan SONG, Lin-chun SHI. Identification of water buffalo horn and its adulterants using COI barcode[J]. Acta Pharmaceutica Sinica, 2017 , 52 (3) : 494 -499 . DOI: 10.16438/j.0513-4870.2016-1020
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Article Info
doi: 10.16438/j.0513-4870.2016-1020
  • Receive Date：2016-10-20
  • Online Date：2026-01-14
  • Published：2017-03-12
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History
  • Received：2016-10-20
  • Revised：2016-12-01
Funding
Affiliations
    1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
    2. College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
    3. Hebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical College, Chengde 067000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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