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Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods
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Chun-ni ZHANG1, Ying-zi WANG1, *, Xin-guang SUN2, Yang ZHAO2, Wei ZHENG3, Wen-hua LI1, Zhen LONG4, Bai-ping MA2, *
Acta Pharmaceutica Sinica | 2017, 52(3) : 456 - 461
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Acta Pharmaceutica Sinica | 2017, 52(3): 456-461
Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods
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Chun-ni ZHANG1, Ying-zi WANG1, *, Xin-guang SUN2, Yang ZHAO2, Wei ZHENG3, Wen-hua LI1, Zhen LONG4, Bai-ping MA2, *
Affiliations
  • 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
  • 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
  • 3. Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • 4. ThermoFisher Scientific Corporation, Beijing 100080, China
Published: 2017-03-12 doi: 10.16438/j.0513-4870.2016-0965
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A new method was developed for the chromatographic fingerprint analysis of Toosendan Fructus by HPLC coupled with the charged aerosol detector (CAD) in the present study. Samples were well separated on an Agilent ZOBAX SB C18 column (4.6 mm×250 mm, 5 μm) by gradient elution using acetonitrile and water containing 0.1% formic acid (v/v) at the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 5 μL. The nitrogen inlet pressure of the charged aerosol detector (CAD) was 35 psi, and the nebulizer chamber temperature was 35℃. In addition, the method of the chromatographic fingerprints combined with multivariate statistical analysis was effective and reasonable lead to an accurate classification of 20 batches of samples from different locations. The results showed that 28 common peaks were observed in the fingerprint and the samples were classified into three clusters. The established method was well validated, and showed high precision, good repeatability, and satisfactory stability. It may serve in the quality control and evaluation of Toosendan Fructus.

Toosendan Fructus  /  HPLC-CAD  /  fingerprint  /  similarity evaluation  /  hierarchical cluster analysis  /  principle components analysis
Chun-ni ZHANG, Ying-zi WANG, Xin-guang SUN, Yang ZHAO, Wei ZHENG, Wen-hua LI, Zhen LONG, Bai-ping MA. Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods[J]. Acta Pharmaceutica Sinica, 2017 , 52 (3) : 456 -461 . DOI: 10.16438/j.0513-4870.2016-0965
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Article Info
doi: 10.16438/j.0513-4870.2016-0965
  • Receive Date：2016-09-30
  • Online Date：2026-01-14
  • Published：2017-03-12
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History
  • Received：2016-09-30
  • Revised：2016-11-23
Funding
Affiliations
    1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
    2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
    3. Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    4. ThermoFisher Scientific Corporation, Beijing 100080, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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