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Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics
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Xiao-yan HE1, Jian-li CHEN1, Huan XIANG2, Yao GAO1, Jun-sheng TIAN1, *, Xue-mei QIN1, *, Guan-hua DU3
Acta Pharmaceutica Sinica | 2017, 52(2) : 245 - 252
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Acta Pharmaceutica Sinica | 2017, 52(2): 245-252
• ORIGINAL ARTICLES •
Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics
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Xiao-yan HE1, Jian-li CHEN1, Huan XIANG2, Yao GAO1, Jun-sheng TIAN1, *, Xue-mei QIN1, *, Guan-hua DU3
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. Physical Education Departments, Shanxi University, Taiyuan 030006, China
  • 3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Published: 2017-02-12 doi: 10.16438/j.0513-4870.2016-0543
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This study was designed to analyze the change of metabolites in the PC12 cells and its medium induced by corticosterone (CORT) and glutamate (Glu) by proton nuclear magnetic resonance (1H NMR) metabolomics. The multivariate statistical analysis was employed to identify the difference between control groups and induced groups, respectively. In addition, metabolite pathway analysis was performed to explore the characteristic of CORT-induced and or Glu-induced PC12 cells depression model, and to provide the references for the selection of in vitro depression models as well as the further understanding of the mechanism on depressive disorders. We found 36 differential metabolites in CORT-induced PC12 cells and medium and 42 in Glu-induced PC12 cells. Furthermore, correlation analysis results show that serine and 2-oxoisoleucine were associated with most differential metabolites in CORT-induced PC12 cells. Lactate and glutathione were significantly correlated to the vast majority of differential metabolites in Glu-induced PC12 cells. We speculated that CORT-induced PC12 cell models may affect the fatty acid metabolism and cell membrane structure, and Glu-induced PC12 cell models may have a difference in the glycolysis and antioxidants.

metabolomics  /  corticosterone  /  glutamate  /  PC12 cell  /  depression
Xiao-yan HE, Jian-li CHEN, Huan XIANG, Yao GAO, Jun-sheng TIAN, Xue-mei QIN, Guan-hua DU. Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics[J]. Acta Pharmaceutica Sinica, 2017 , 52 (2) : 245 -252 . DOI: 10.16438/j.0513-4870.2016-0543
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Article Info
doi: 10.16438/j.0513-4870.2016-0543
  • Receive Date：2016-06-04
  • Online Date：2026-01-14
  • Published：2017-02-12
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  • Received：2016-06-04
  • Revised：2016-08-30
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Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. Physical Education Departments, Shanxi University, Taiyuan 030006, China
    3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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