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The molecular identification of Bupleurum medicinal species and the quality investigation of Bupleuri Radix
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Bo-chuan YUAN1, Wen-dong LI2, Yong-sheng MA1, Shan ZHOU1, Lin-feng ZHU1, Rui-chao LIN3, *, Ying LIU1
Acta Pharmaceutica Sinica | 2017, 52(1) : 162 - 171
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Acta Pharmaceutica Sinica | 2017, 52(1): 162-171
• ORIGINAL ARTICLES •
The molecular identification of Bupleurum medicinal species and the quality investigation of Bupleuri Radix
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Bo-chuan YUAN1, Wen-dong LI2, Yong-sheng MA1, Shan ZHOU1, Lin-feng ZHU1, Rui-chao LIN3, *, Ying LIU1
Affiliations
  • 1. School of Life Science, Beijing University of Chinese Medicine, Beijing 100102, China
  • 2. Beijing Institute for Drug Control, Beijing 102206, China
  • 3. Traditional Chinese Medicine Quality Evaluation Research Center, Beijing University of Chinese Medicine, Beijing 100102, China
Published: 2017-01-12 doi: 10.16438/j.0513-4870.2016-0539
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Bupleuri Radix is one of the most frequently used herbal medicines in China with a 2000-year medicinal history. However, the use of Bupleuri Radix is very confused. Twenty-five species and eight varieties of Bupleurum have been used as Bupleuri Radix in different regions of China. It is very difficult to identify these Bupleurum species using traditional morphological method. In order to establish a fast and effective method to identify these Bupleurum species, we collected 168 Bupleurum medicinal plants from 14 populations of 9 provinces, and amplified their ITS sequences. 168 ITS sequences with a full length of 600-606 bp were obtained. DNAMAN analyzing results showed that 86 variable sites were present in these sequences and 19 haplotypes (TH1-TH19) were determined. After calculating K2P distance and analyzing an NJ tree, we established a molecular identification method based on ITS sequence. Using this method,52 samples of Bupleuri Radix were identified successfully. Furthermore, we tested saikosaponin a, c, d contents in these Bupleuri Radix by HPLC and analyzed the results by ANOVA and LSD T test to evaluate the quality of Bupleuri Radix. This method is significant for effective identification of Bupleurum medicinal plants, and quality control of Bupleuri Radix in the market.

Bupleurum  /  ITS  /  molecular identification  /  saikosaponins  /  quality evaluation
Bo-chuan YUAN, Wen-dong LI, Yong-sheng MA, Shan ZHOU, Lin-feng ZHU, Rui-chao LIN, Ying LIU. The molecular identification of Bupleurum medicinal species and the quality investigation of Bupleuri Radix[J]. Acta Pharmaceutica Sinica, 2017 , 52 (1) : 162 -171 . DOI: 10.16438/j.0513-4870.2016-0539
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Article Info
doi: 10.16438/j.0513-4870.2016-0539
  • Receive Date：2016-06-03
  • Online Date：2026-01-14
  • Published：2017-01-12
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History
  • Received：2016-06-03
  • Revised：2016-06-26
Funding
Affiliations
    1. School of Life Science, Beijing University of Chinese Medicine, Beijing 100102, China
    2. Beijing Institute for Drug Control, Beijing 102206, China
    3. Traditional Chinese Medicine Quality Evaluation Research Center, Beijing University of Chinese Medicine, Beijing 100102, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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