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The present quality control methods of traditional Chinese medicine (TCM), such as morphological identification, microscopic identification, physicochemical analysis, and biological activity testing, cannot fully meet the requirements of TCM quality control. Based on the characteristics of digital PCR technology, a new molecular quantitative analysis technology named Digital Dancao molecular detection technology was established, which used the ratio of specific sequence copy number to internal reference sequence copy number (R value) as the quality control indicator. This technology can quantitatively analyze the adulteration of medicinal herbs, monitor the feeding amount and production process of preparation. It conforms to the clinical characteristics of TCM which using herbal dosage, and can be used for quantitative testing of TCM products from herbs to preparation. Digital Dancao molecular detection technology provides a new solution for the problem of quality monitoring of TCM.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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