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To establish the HPLC characteristic chromatogram for Gujing Maisiha tablets，and evaluate the quality using chemical pattern recognition.
The Welch Xtimate C18 column (250 mm × 4.6 mm, 5 μm) was used and the mobile phases were acetonitrile (A) and 0.1% formic acid (B) in gradient elution (0-10 min，5%A→15%A；10-20 min，15%A；20-40 min，15%A→40%A；40-65 min，40%A→70%A；65-66 min，70%A→90%A；66-80 min，90%A；80-81 min，90%A→5%A；81-85 min，5%A). The detection wavelength was 254 nm. The column temperature was 35 ℃ and the flow rate was 1.0 mL·min-1. Gujing Maisiha tablets were analyzed to construct characteristic chromatogram. The quality of Gujing Maisiha tablets was assessed through similarity evaluation，cluster analysis，principal component analysis (PCA)，and partial least squares discriminant analysis (PLS-DA).
The characteristic chromatogram of Gujing Maisiha tablets was established，which contained the twenty-eight distinct peaks，and seven compounds identified as gallic acid，chlorogenic acid，picrocrocin，isoquercitrin，crocin-Ⅰ，crocin-Ⅱ，eugenol. The similarities for the ten batches of Gujing Maisiha tablets were more than 0.98，with no significant difference observed. Cluster analysis segregated the samples into two distinct groups according to the Euclidean distance of 10，which was consistent with the results of PCA analysis. PLS-DA analysis screened out five components from Flos Caryophylli，Rosae Rugosae Flos，Olibanum that may cause differences between samples.
The method of HPLC characteristic chromatogram of Gujing Maisiha tablets is effective，feasible and reproducible，which can provide reference for the study of quality standard of this product.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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