PDF
Full
Outline
Objective: To establish the HPLC fingerprint of Gardeniae Fructus before and after processing, and to study the relationship between the fingerprint and antioxidant activity. Methods: Agilent 1200 high-performance liquid chromatograph was used, and the column was Agilent ZORBAX SB-C18 (250 mm×4.6 mm, 5 μm). The mobile phase system was 0.1% formic acid water-methanol solution. The ultraviolet detection wavelength was 254 nm. The column temperature was 25 ℃. The extraction solvent of the test solution was 70% methanol solution.The injection volume was 5 μL. The flow rate was 1 mL · min-1, and the fingerprints of six batches of gardenia before and after processing were established, and the antioxidant activity of gardenia before and after processing was evaluated by DPPH method, and the spectral effect relationship was studied by grey correlation analysis and partial least squares regression analysis. Results: 23 common peaks were identified in the fingerprint, and five chromatographic peaks were identified by comparison of reference substances, including geniposidic acid (peak 3), genipin gentiobiside (peak 9), gardenside (peak 11), crocetin I (peak 21), and crocetin Ⅱ (peak 22). The mean half inhibitory concentration (IC50) of DPPH radical in 5 different processed products (raw gardenia, fried gardenia,wine gardenia, gardenia charcoal and ginger gardenia) were 0.25 mg · mL-1, 28.61 mg · mL-1, 6.34 mg · mL-1,11.79 mg · mL-1, and 0.68 mg · mL-1, respectively. Combined with the three statistical methods, peaks 3, 9, 11, 21,and 22 were the common peaks associated with DPPH radical scavenging. Conclusion: The antioxidant activity of 6 batches of Gardeniae Fructus before and after processing is the result of the interaction of multiple components, and peaks 3, 9, 11, 21, and 22 are closely related to antioxidant activity. The results of this study provide a reference for the basic research and clinical application of antioxidant substances in Gardeniae Fructus before and after processing.
PDF
113
52
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
