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Research progress on extraction, isolation, structural identification and analytical methods of oligosaccharides from Chinese herbal medicine*
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Hong GUO1, Rui YAO1, Jing FAN1, Xiao-han GUO1, Jia CHEN1, Bao-zhong DUAN2, Ying WANG1, **, Jian-bo YANG1, **, Wei CAI3, Wen-guang JING1, Xian-long CHENG1, Feng WEI1
Chinese Journal of Pharmaceutical Analysis | 2025, 45(3) : 361 - 391
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Chinese Journal of Pharmaceutical Analysis | 2025, 45(3): 361-391
• Review & Monography •
Research progress on extraction, isolation, structural identification and analytical methods of oligosaccharides from Chinese herbal medicine*
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Hong GUO1, Rui YAO1, Jing FAN1, Xiao-han GUO1, Jia CHEN1, Bao-zhong DUAN2, Ying WANG1, **, Jian-bo YANG1, **, Wei CAI3, Wen-guang JING1, Xian-long CHENG1, Feng WEI1
Affiliations
  • 1. National Institutes for Food and Drug Control, Beijing 100050, China
  • 2. College of Pharmaceutical Science,Dali University, Dali 671000, China
  • 3. Hunan University of Medicine, Huaihua 418000, China
Published: 2025-03-31 doi: 10.16155/j.0254-1793.2024-1061
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Oligosaccharides are a class of bioactive components abundantly found in Chinese herbal medicine. They have clinical effects such as antidepressant and anti-Alzheimer’s disease activities, as well as pharmacological activities such as hypoglycemic, laxative, and immune-enhancing effects. The bioactivity of oligosaccharides is closely related to their structure. However, due to the diversity of glycosidic bond configurations, monosaccharide compositions and linkage patterns, it is difficult to accurately determine their structures by conventional analytical methods. Establishing rapid and reliable analytical methods remains a key challenge in carbohydrate research.Although studies on oligosaccharides are increasing, there is a lack of comprehensive reviews on the structure and analytical methods of oligosaccharides in Chinese herbal medicine. This paper systematically reviewed the research progress over the past decade on extraction, separation, structure identification and analytical methods of oligosaccharides in Chinese herbal medicine by consulting domestic and foreign literatures. This review aims to contribute to the development of evaluation method of traditional Chinese medicine based on oligosaccharide components, and provide reference for their application in the quality control of traditional Chinese medicine.

Chinese herbal medicine  /  oligosaccharides  /  extraction and separation  /  structure identification  /  analytical methods  /  quality control
Hong GUO, Rui YAO, Jing FAN, Xiao-han GUO, Jia CHEN, Bao-zhong DUAN, Ying WANG, Jian-bo YANG, Wei CAI, Wen-guang JING, Xian-long CHENG, Feng WEI. Research progress on extraction, isolation, structural identification and analytical methods of oligosaccharides from Chinese herbal medicine*[J]. Chinese Journal of Pharmaceutical Analysis, 2025 , 45 (3) : 361 -391 . DOI: 10.16155/j.0254-1793.2024-1061
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Year 2025 volume 45 Issue 3
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doi: 10.16155/j.0254-1793.2024-1061
  • Receive Date：2024-08-20
  • Online Date：2026-03-17
  • Published：2025-03-31
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  • Received：2024-08-20
Funding
Affiliations
    1. National Institutes for Food and Drug Control, Beijing 100050, China
    2. College of Pharmaceutical Science,Dali University, Dali 671000, China
    3. Hunan University of Medicine, Huaihua 418000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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