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Rapid authentication of Eupolyphaga sinensis by loop-mediated isothermal amplification
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Wei-tao CHEN1, Yang-xue LI1, Jie-yi JIANG1, Can-hui XIE2, De-zheng JIA2, Xiao-tong LIU3, Hui-na LIAO3, Su-mei LI1, **
Chinese Journal of Pharmaceutical Analysis | 2024, 44(9) : 1529 - 1534
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Chinese Journal of Pharmaceutical Analysis | 2024, 44(9): 1529-1534
• Standard Deliberation •
Rapid authentication of Eupolyphaga sinensis by loop-mediated isothermal amplification
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Wei-tao CHEN1, Yang-xue LI1, Jie-yi JIANG1, Can-hui XIE2, De-zheng JIA2, Xiao-tong LIU3, Hui-na LIAO3, Su-mei LI1, **
Affiliations
  • 1.Guangdong Second Traditional Chinese Medicine Hospital (Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine), Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong 510095, China
  • 2.The School of the Fifth Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
  • 3.School of Pharmacy, Guangzhou Xinhua University, Guangzhou 510520, China
Published: 2024-09-30 doi: 10.16155/j.0254-1793.2024-0035
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Objective: To establish an authentication method for Eupolyphaga sinensis based on loop-mediated isothermal amplification (LAMP). Methods: Two pairs of primers （outer primer DB-F3, DB-B3 and inner primer DB-FIP, DB-BIP） were designed according to Eupolyphaga sinensis COⅠspecific loci. The reaction system containing template, primers, Bst DNA polymerase and methyl red-phenol red indicator amplified at 63 ℃ for 90 min. The LAMP results were determined by visual observation and compared with DNA barcoding and polymerase chain reaction (PCR) methods to investigate the specificity and sensitivity. Results: All 11 batches of the authentic Eupolyphaga sinensis sample tubes colour changed from purple red to orange yellow, while sample tubes of 1 batch of Steleophaga plancyi, 9 batches Opisthoplatia orientalis and 1 batch of the adulterant, Cybister tripunctatus orientalis, remained purple red after the reaction. The LAMP results were consistent with those of DNA barcoding and PCR. The detection limit of LAMP was 0.984 ng·mL-1. Conclusion: The established LAMP method is accurate, sensitive and easy to operate, low equipment required, and can be applied to the rapid screening of the authentication of Eupolyphaga sinensis.

Eupolyphaga sinensis  /  loop-mediated isothermal amplification  /  DNA barcoding  /  polymerase chain reaction  /  authentication of Chinese medicinal materials  /  visual check  /  rapid identification
Wei-tao CHEN, Yang-xue LI, Jie-yi JIANG, Can-hui XIE, De-zheng JIA, Xiao-tong LIU, Hui-na LIAO, Su-mei LI. Rapid authentication of Eupolyphaga sinensis by loop-mediated isothermal amplification[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44 (9) : 1529 -1534 . DOI: 10.16155/j.0254-1793.2024-0035
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Year 2024 volume 44 Issue 9
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Article Info
doi: 10.16155/j.0254-1793.2024-0035
  • Receive Date：2024-01-18
  • Online Date：2026-03-17
  • Published：2024-09-30
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History
  • Received：2024-01-18
Funding
Affiliations
    1.Guangdong Second Traditional Chinese Medicine Hospital (Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine), Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong 510095, China
    2.The School of the Fifth Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
    3.School of Pharmacy, Guangzhou Xinhua University, Guangzhou 510520, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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