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To explore the difference of chemical composition between the bulbus of Lilium lancifolium Thunb. and L. brownii F. E. Brown var. viridulum Baker，the chemical profile of Lilii Bulbus was acquired by ultra-high performance liquid chromatography with quadrupole-time of flight mass spectrometry (UPLC-Q TOF MS)，then the components of all samples was analyzed by chemometrics combined with molecular networking.
The Agilent poroshell 120 EC-C18 column (100 mm×2.1 mm，2.7 μm) was adopt，and the mobile phase was acetonitrile -0.1% formic acid aqueous solution with gradient elution. The flow rate was 0.3 mL·min-1，the column temperature was 30 ℃ and the injection volume was 1 μL. The mass spectra were acquired in the positive and negative modes in the mass range of m/z 80-1 100. Principal component analysis (PCA)，partial least squares-discriminant analysis (OPLS-DA) and single factor analysis were used for screening the differential components. Then GNPS molecular network was created according to the similarity of MS/MS fragmentation modes. Cytoscape 3.7.2 software was used to screen molecular clusters with similar structures.
Phenolic acid glycerides，alkaloids and steroid saponins were screened as the differential components groups. Among these components，the bulbus of Lilium lancifolium Thunb. was rich in steroid saponins，while the bulbus of L. brownii F. E. Brown var. viridulum Baker was rich in alkaloids. Besides，31 components，including 18 phenolic acid glycerides，7 alkaloids and 6 steroid saponins were identified in the three differential components groups.
This method can provide reference data for the quality control and pharmacodynamic substances of Lilii Bulbus，and provide reference for the rapid qualitative analysis of chemical components of traditional Chinese medicine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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