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To study the toxicity of heavy metal cadmium and screen detoxification drugs base on Caenorhabditis elegans.
The microfluidics chip technology was used as the drug screening platform. The 96-well plate exposure pretest was used, and K-medium blank group was set up. The exposure group consisted of nematodes exposed to heavy metal cadmium at the environmental relevant concentration of 0.25-15.0 μg·mL after homogenization. Vitamin C and calcium disodium ethylenediamine tetraacetate were selected as detoxification group. （10±2） nematodes were added into each orifice plate. The number of nematodes was observed and recorded under a microscope, and paid attention to the changes in the structure of the vulva. The experimental data were processed and analyzed by origin 2019b software.
Compared with blank group, the fatality rates of C. elegans in exposed group were respectively 0%, 1.67%, 4.76%, 67.46%, 100% and 100% at 0.25, 1.50, 5.0, 10.0, 12.5, 15.0 μg·mL-1 concentration, compared with blank group. The structure of the nematode vulva was deformed, from slight protrusion to severe protrusion, and even a tuberous protrusion, and finally ruptured. The mortality of detoxification group was reduced to different degrees compared with exposure group.
Compared with blank control group, with the increase of cadmium exposure concentration and exposure time, cadmium can inhibit the survival of C. elegans, and damage the structure of C. elegans vulva to varying degrees. The results of detoxification group showed that vitamin C had little detoxification effect on cadmium. The complexation of calcium disodium ethylenediamine tetraacetate with cadmium could prolong the survival time of C. elegans to a large extent.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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