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Screening and validation of anti-vascular disease active components from Semen raphani based on HUVEC cell membrane chromatography*
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Hua-ni LI1, 2, Chang-he LIU1, 2, **, Dan-dan JIAN3, Sheng-hu CHEN3, Wen-jing GE1, 2, Xue-xia ZHANG1, 2
Chinese Journal of Pharmaceutical Analysis | 2024, 44(6) : 929 - 937
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Chinese Journal of Pharmaceutical Analysis | 2024, 44(6): 929-937
• Ingredient Analysi •
Screening and validation of anti-vascular disease active components from Semen raphani based on HUVEC cell membrane chromatography*
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Hua-ni LI1, 2, Chang-he LIU1, 2, **, Dan-dan JIAN3, Sheng-hu CHEN3, Wen-jing GE1, 2, Xue-xia ZHANG1, 2
Affiliations
  • 1.Henan Integrative Medicine Hospital/Henan Provincial Academy of Traditional Chinese Medicine and Pharmacy, Zhengzhou 450004, China
  • 2.Henan Engineering Research Center of Traditional Chinese Medicine Preparation, Zhengzhou 450004, China
  • 3.Pharmaceutical College of Henan University, Kaifeng 475004, China
Published: 2024-06-30 doi: 10.16155/j.0254-1793.2024.06.02
Outline
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Objective:

To establish a method for determination of potential anti-vascular disease active components of Semen raphani based on cell membrane chromatographic (CMC) model of human umbilical vein cell (HUVEC).

Methods:

The HUVEC cell membrane chromatography coupled with HPLC-ESI IT TOF MS system were used to screen the active components of Semen raphani. The selected active components were further applied to ox-LDL induced HUVEC to verify their protective effects.

Results:

Two retained components were selected from Semen raphani by this method. One component was identified as erucinic acid by comparing with the reference material. Compared with the model group, the cell survival rates of the erucinic acid pretreatment groups increased significantly. The amount of ICAM-1 and VCAM-1 decreased in a dose-dependent manner. Bcl-2 protein levels decreased and Bax protein levels increased, with statistical significance (P<0.05).

Conclusion:

The method can rapidly obtain active ingredients from complex traditional Chinese medicines. It provides a reference for the application of cell membrane chromatography and the development of Semen raphani.

Semen raphani  /  cell membrane chromatography  /  erucinic acid  /  human umbilical vein cell
Hua-ni LI, Chang-he LIU, Dan-dan JIAN, Sheng-hu CHEN, Wen-jing GE, Xue-xia ZHANG. Screening and validation of anti-vascular disease active components from Semen raphani based on HUVEC cell membrane chromatography*[J]. Chinese Journal of Pharmaceutical Analysis, 2024 , 44 (6) : 929 -937 . DOI: 10.16155/j.0254-1793.2024.06.02
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Year 2024 volume 44 Issue 6
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Article Info
doi: 10.16155/j.0254-1793.2024.06.02
  • Receive Date：2023-12-01
  • Online Date：2026-03-13
  • Published：2024-06-30
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History
  • Received：2023-12-01
Funding
Affiliations
    1.Henan Integrative Medicine Hospital/Henan Provincial Academy of Traditional Chinese Medicine and Pharmacy, Zhengzhou 450004, China
    2.Henan Engineering Research Center of Traditional Chinese Medicine Preparation, Zhengzhou 450004, China
    3.Pharmaceutical College of Henan University, Kaifeng 475004, China
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https://castjournals.cast.org.cn/joweb/ywfxzz/EN/10.16155/j.0254-1793.2024.06.02
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表12种不同金属材料的力学参数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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